Western Blot (WB)
(Western blot analysis of Hela whole cell lysates, using EBAG9 Antibody. The lane on the left is treated with the antigen-specific peptide.)
Rabbit EBAG9 Polyclonal Antibody | anti-EBAG9 antibody
EBAG9 Antibody
Gene Names
EBAG9; EB9; PDAF
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
EBAG9; Polyclonal Antibody; EBAG9 Antibody; BAG9; Cancer associated surface antigen; Cancer associated surface antigen RCAS1; Cancer-associated surface antigen RCAS1; EB9; EBAG 9; Estrogen receptor binding fragment associated gene 9 protein; Estrogen receptor binding site associated antigen 9; estrogen receptor binding site associated; antigen; 9; Estrogen receptor-binding fragment-associated gene 9 protein; PDAF; RCAS 1; RCAS1; RCAS1_HUMAN; Receptor binding cancer antigen expressed on SiSo cells; Receptor-binding cancer antigen expressed on SiSo cells; anti-EBAG9 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
EBAG9 antibody detects endogenous levels of total EBAG9
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
213
Applicable Applications for anti-EBAG9 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human EBAG9
Subcellular Location
Golgi Apparatus Membrane. According to PubMed:10426319, it also exists as a soluble form which has the same biological activities. The existence of such soluble form is however uncertain.
Tissue Specificity
Widely expressed. Expressed in ovary, testis, prostate, thymus, muscle and heart, but not in small intestine, colon, lymph nodes, or peripherical blood lymphocytes. The protein is not detected in any of the above organs.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (89%), Chicken (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of Hela whole cell lysates, using EBAG9 Antibody. The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of Hela whole cell lysates, using EBAG9 Antibody. The lane on the left is treated with the antigen-specific peptide.)
Immunohistochemistry (IHC)
(MBS9607359 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9607359 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-EBAG9 antibody
Description: This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor, which binds to the estrogen-responsive element found in the 5'-flanking region of this gene. The encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Alternate splicing results in multiple transcript variants. A pseudogene of this gene has been defined on chromosome 10.
Function: May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases.
Subunit Structure: Homodimer.
Similarity: The coiled coil domain is necessary for the homodimerization.
Function: May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases.
Subunit Structure: Homodimer.
Similarity: The coiled coil domain is necessary for the homodimerization.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 24 kDa
Predicted: 25 kDa
Predicted: 25 kDa
NCBI Official Full Name
receptor-binding cancer antigen expressed on SiSo cells
NCBI Official Synonym Full Names
estrogen receptor binding site associated, antigen, 9
NCBI Official Symbol
EBAG9
NCBI Official Synonym Symbols
EB9; PDAF
NCBI Protein Information
receptor-binding cancer antigen expressed on SiSo cells
UniProt Protein Name
Receptor-binding cancer antigen expressed on SiSo cells
Protein Family
UniProt Gene Name
EBAG9
UniProt Synonym Gene Names
RCAS1
NCBI Description
This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor, which binds to the estrogen-responsive element found in the 5'-flanking region of this gene. The encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Alternate splicing results in multiple transcript variants. A pseudogene of this gene has been defined on chromosome 10. [provided by RefSeq, Jul 2013]
Uniprot Description
May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases.
Research Articles on EBAG9
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Product Notes
The EBAG9 ebag9 (Catalog #AAA9607359) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EBAG9 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's EBAG9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the EBAG9 ebag9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EBAG9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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