anti-Rat EAT-4 Polyclonal Antibody | anti-EAT-4 antibody
Anti - EAT-4 (Vesicular Type Transporters for Glutamate, VGLUT)
Reactivity
Rat
Applications
ELISA, Western Blot
Purity
Serum
Synonyms
EAT-4; Polyclonal Antibody; Anti - EAT-4 (Vesicular Type Transporters for Glutamate; VGLUT); EAT-4 (Vesicular Type Transporters for Glutamate; anti-EAT-4 antibody
Host
Host: Rabbit
Source: Rat
Source: Rat
Reactivity
Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
The peptide has no significant sequence with mammalian VGLUT1/2 or other NaPi-related (Type-1 to Type-III) transporters. Antibody crossreactivity in various species is not established. Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking (use 5-10 ug per 1 ul of antiserum or 1 ug of aff pure IgG) to confirm antibody specificity.
Purity/Purification
Serum
Form/Format
Serum
Supplied as a liquid, neat serum, 40% glycerol.
Supplied as a liquid, neat serum, 40% glycerol.
Sequence Length
576
Applicable Applications for anti-EAT-4 antibody
ELISA, Western Blot
Application Notes
Suitable for use in ELISA, Western Blotting. Other applications not tested.
Dilution: Western Blot: 1:1000-1:5000 for antiserum and 1-10ug/ml for affinity pure antibody using Chemiluminescence technique. EAT-4 is ~65kD protein.
ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure. Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Dilution: Western Blot: 1:1000-1:5000 for antiserum and 1-10ug/ml for affinity pure antibody using Chemiluminescence technique. EAT-4 is ~65kD protein.
ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure. Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Immunogen
An 18 AA synthetic peptide witin the N-terminus of C. elegans EAT-4 was synthesized, conjugated to KLH
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-EAT-4 antibody
Glutamate is the main excitatory neurotransmitter in the brain. To date five glutamate Transporters have been cloned: GLAST (EAAT1), GLT1 (EAAT2), EAAC1 (EAAT3), EAAT4, and EAAT5. These transporters are believed to be critical in reducing potentially toxic extracellular concentration of glutamate by rapid uptake into nerve terminals and glial cells. Most recently, vesicular type transporters for glutamate, termed VGLUTs (VGLUT1/BNPI, VGLUT2/DNPI, and EAT-4), have been cloned and characterized that are related to phosphate transporters. Although neurons exhibit Na-dependent Pi transport, the biological role of Pi uptake is not clear. Proximal tubules in the kidney reabsorb Pi in the glomeruli by the action of a group of phosphate transporters (Type1-NaPi related, type2-NaPi-2 related, and type 3-viral receptor-related. These receptors show weak (~20% identity) between various subtypes. A distinct type of brain specific Na+-dependent phosphate (Pi) transporter (BNPI), originally characterized as a plasma membrane transporter has been localized in a subset of glutamatergic neurons (amygdla,cereberal cortex, and hippocmaplus) and identified as VGLUT1 (rat and human 560 aa; ~60kD, ~30% homology with type-1 Pi-transporters). VGLUT1 shows strong sequence homology to EAT-4, a C. elegans protein that appears to have specific presynaptioc role in glutamtergic transmission. Loss of function mutations in EAT-4 affects multiple glutamregic neurotransmission pathways. EAT4 encodes a protein of 563 aa (~48% identity with BNPI and ~30% identity with known NaPi-transporters found in mammalian kidney). Like BNPI, EAT-4 is also expressed in glutamatergic neurons.
Product Categories/Family for anti-EAT-4 antibody
References
• 1. Lee RYN et al (1999) J. Neurosci. 19:159-167
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
63,109 Da
NCBI Official Full Name
EATing: abnormal pharyngeal pumping family member (eat-4)
NCBI Official Symbol
eat-4
NCBI Protein Information
EATing: abnormal pharyngeal pumping family member (eat-4)
UniProt Protein Name
Probable vesicular glutamate transporter eat-4
Protein Family
UniProt Gene Name
eat-4
UniProt Entry Name
EAT4_CAEEL
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Product Notes
The EAT-4 eat-4 (Catalog #AAA612098) is an Antibody produced from Host: Rabbit Source: Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti - EAT-4 (Vesicular Type Transporters for Glutamate, VGLUT) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's EAT-4 can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. Suitable for use in ELISA, Western Blotting. Other applications not tested. Dilution: Western Blot: 1:1000-1:5000 for antiserum and 1-10ug/ml for affinity pure antibody using Chemiluminescence technique. EAT-4 is ~65kD protein. ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure. Control peptide can be used to coat ELISA plates at 1ug/ml. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the EAT-4 eat-4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EAT-4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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