Western Blot (WB)
(Western blot analysis of anti-EARS2 Pab in HL60 cell line lysates (35ug/lane).EARS2 (arrow) was detected using the purified Pab.)
Rabbit anti-Human EARS2 Polyclonal Antibody | anti-EARS2 antibody
EARS2, CT (Probable Glutamyl-tRNA Synthetase, Mitochondrial, Glutamate-tRNA Ligase, GluRS, KIAA1970)
Purified by ammonium sulfate precipitation.
Purified by ammonium sulfate precipitation.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:10-1:50
Western Blot (WB)
(Western blot analysis of anti-EARS2 Pab in HL60 cell line lysates (35ug/lane).EARS2 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of anti-EARS2 Pab in HL60 cell line lysates (35ug/lane).EARS2 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with EARS2 antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with EARS2 antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
NCBI and Uniprot Product Information
NCBI Description
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene belongs to the class-I aminoacyl-tRNA synthetase family and catalyzes the attachment of glutamate to tRNA(Glu). Alternative splicing results in multiple transcript variants. [provided by RefSeq]
Uniprot Description
EARS2: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu). Belongs to the class-I aminoacyl-tRNA synthetase family.
Protein type: Translation; Mitochondrial; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; EC 6.1.1.17; Ligase
Chromosomal Location of Human Ortholog: 16p12.2
Cellular Component: mitochondrion; mitochondrial matrix
Molecular Function: glutamate-tRNA(Gln) ligase activity; glutamate-tRNA ligase activity; ATP binding; tRNA binding
Biological Process: tRNA aminoacylation for protein translation; glutamyl-tRNA aminoacylation; gene expression
Disease: Combined Oxidative Phosphorylation Deficiency 12
Research Articles on EARS2
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Product Notes
The EARS2 ears2 (Catalog #AAA623737) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EARS2, CT (Probable Glutamyl-tRNA Synthetase, Mitochondrial, Glutamate-tRNA Ligase, GluRS, KIAA1970) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EARS2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Dilution: ELISA: 1:1,000 Western Blot: 1:50-1:100 Immunohistochemistry: 1:10-1:50. Researchers should empirically determine the suitability of the EARS2 ears2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EARS2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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