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Western Blot (WB) (Western BlotPositive WB detected in: Mouse kidney tissueAll lanes: EAF2 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 29,15 KDaObserved band size: 45 KDa)

Rabbit anti-Human, Mouse EAF2 Polyclonal Antibody | anti-EAF2 antibody

EAF2 Antibody

Gene Names
EAF2; U19; BM040; TRAITS
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Synonyms
EAF2; Polyclonal Antibody; EAF2 Antibody; ELL-associated factor 2; Testosterone-regulated apoptosis inducer and tumor suppressor protein; TRAITS; BM-040; anti-EAF2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
130
Applicable Applications for anti-EAF2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Species
Human
Immunogen
Recombinant human ELL-associated factor 2 protein
Conjugate
Non-conjugated
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-66369 / sc-66371 / sc-66374 / sc-66375
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Mouse kidney tissueAll lanes: EAF2 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 29,15 KDaObserved band size: 45 KDa)

Western Blot (WB) (Western BlotPositive WB detected in: Mouse kidney tissueAll lanes: EAF2 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 29,15 KDaObserved band size: 45 KDa)

Immunohistochemistry (IHC)

(IHC image of MBS7045417 diluted at 1:1000 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunohistochemistry (IHC) (IHC image of MBS7045417 diluted at 1:1000 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunohistochemistry (IHC)

(Immunofluorescent analysis of HepG-2 cells using MBS7045417 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Immunohistochemistry (IHC) (Immunofluorescent analysis of HepG-2 cells using MBS7045417 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Related Product Information for anti-EAF2 antibody
Acts as a transcriptional transactivator of TCEA1 elongation activity (By similarity). Acts as a transcriptional transactivator of ELL and ELL2 elongation activities. Potent inducer of apoptosis in prostatic and non-prostatic cell lines. Inhibits prostate tumor growth in vivo.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
14,091 Da
NCBI Official Full Name
ELL-associated factor 2 isoform 2
NCBI Official Synonym Full Names
ELL associated factor 2
NCBI Official Symbol
EAF2
NCBI Official Synonym Symbols
U19; BM040; TRAITS
NCBI Protein Information
ELL-associated factor 2
UniProt Protein Name
ELL-associated factor 2
Protein Family
UniProt Gene Name
EAF2
UniProt Synonym Gene Names
TRAITS
UniProt Entry Name
EAF2_HUMAN

Uniprot Description

EAF2: Acts as a transcriptional transactivator of TCEA1 elongation activity. Acts as a transcriptional transactivator of ELL and ELL2 elongation activities. Potent inducer of apoptosis in prostatic and non-prostatic cell lines. Inhibits prostate tumor growth in vivo. Belongs to the EAF family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 3q13.33

Cellular Component: nucleoplasm; transcription elongation factor complex

Molecular Function: protein binding

Biological Process: positive regulation of transcription from RNA polymerase II promoter

Research Articles on EAF2

Similar Products

Product Notes

The EAF2 eaf2 (Catalog #AAA7045417) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EAF2 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EAF2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Researchers should empirically determine the suitability of the EAF2 eaf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EAF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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