Rabbit EAAT1 Polyclonal Antibody | anti-EAAT1 antibody

EAAT1 Antibody FITC-Conjugated

Cat. Num. AAA541461

• Gene Names: Slc1a3; Gmt1; Eaat1; GLAST; GLU-T; GluT-1; MGluT1; GLAST-1; AI504299; B430115D02Rik
• Reactivity: Human, Monkey, Mouse, Rat
• Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
• Synonyms: EAAT1; Polyclonal Antibody; EAAT1 Antibody FITC-Conjugated; EA6; Excitatory amino acid transporter 1; FLJ25094; GLAST; Glial high affinity glutamate transporter; glutamate/aspartate transporter; high affinity; sodium-dependent; High affinity neuronal glutamate transporter; Slc1a3; Sodium dependent glutamate/aspartate transporter; Solute carrier family 1 member 3 antibody; anti-EAAT1 antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Will not cross react with EAAT2 protein.
• Form/Format: FITC-Conjugated
• Concentration: 0.61 ug/ul in antibody stabilization buffer (varies by lot)
• Sequence Length: 543

• Applicable Applications for anti-EAAT1 antibody: ELISA (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Dot Blot: 1:10,000; ELISA: 1:10,000; Immunohistochemistry: 1:100; Immunoprecipitation: 1:200; Western Blot: 1:500
• Immunogen: Synthetic peptide corresponding to C terminal amino acids 480-543 of Mouse EAAT1 protein. The selected peptide was post-synthetically modified to achieve highest antigenicity before coupling to carrier protein using heterobifunctional cross linker for immunogen preparation.; Sequence: KPYQLIAQDNEPEKPVADSETK
• Expression: Purkinje cell layer in cerebellum, lung, spleen, skeletal muscle and testis.
• Molecular Function: high-affinity glutamate transmembrane transporter activity;sodium:dicarboxylate symporter activity
• Structure: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A3 subfamily has a Transmembrane helix.
• Subcellular Location: Plasma membrane; Cell surface; Fibril; Neuronal cell body
• Preparation and Storage: -20 degree C for long term storage

Related Product Information for anti-EAAT1 antibody

FITC-Conjugated Excitatory Amino Acid Transporter 1 Antibody
Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.

NCBI and Uniprot Product Information

• NCBI GI #: 24233554
• NCBI GeneID: 20512
• NCBI Accession #: NP_683740.1
• NCBI GenBank Nucleotide #: NM_148938.3
• UniProt Accession #: P56564; Q99P53
• Molecular Weight: 59,622 Da
• NCBI Official Full Name: excitatory amino acid transporter 1
• NCBI Official Synonym Full Names: solute carrier family 1 (glial high affinity glutamate transporter), member 3
• NCBI Official Symbol: Slc1a3
• NCBI Official Synonym Symbols: Gmt1; Eaat1; GLAST; GLU-T; GluT-1; MGluT1; GLAST-1; AI504299; B430115D02Rik
• NCBI Protein Information: excitatory amino acid transporter 1
• UniProt Protein Name: Excitatory amino acid transporter 1
• UniProt Gene Name: Slc1a3
• UniProt Synonym Gene Names: Eaat1; Gmt1; GLAST-1
• UniProt Entry Name: EAA1_MOUSE

Uniprot Description

SLC1A3: Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia. Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Transporter; Membrane protein, multi-pass; Transporter, SLC family; Membrane protein, integral

Cellular Component: basolateral plasma membrane; cell projection; cell soma; cell surface; dendritic spine; fibril; integral to membrane; membrane; mitochondrial inner membrane; neuron projection; plasma membrane; synapse

Molecular Function: amino acid binding; glutamate binding; high-affinity glutamate transmembrane transporter activity; L-glutamate transmembrane transporter activity; protein binding; sodium:dicarboxylate symporter activity; symporter activity

Biological Process: auditory behavior; cranial nerve development; dicarboxylic acid transport; gamma-aminobutyric acid biosynthetic process; glutamate biosynthetic process; glutamate metabolic process; L-glutamate import; L-glutamate transport; malate-aspartate shuttle; neuromuscular process controlling balance; neuron morphogenesis during differentiation; positive regulation of defense response to virus by host; positive regulation of synaptic transmission; response to amino acid stimulus; response to antibiotic; response to drug; response to inorganic substance; response to light stimulus; response to wounding; sensory perception of sound; transport