Western Blot (WB)
(Western Blot analysis of RetSat expression in transfected 293T cell line by RetSat polyclonal antibody. Lane 1: RetSat transfected lysate (22.55kD). Lane 2: Non-transfected lysate.)
Mouse anti-Human RetSat Polyclonal Antibody | anti-RETSAT antibody
RetSat (Retinol Saturase, All-trans-retinol 13,14-reductase, All-trans-13,14-dihydroretinol Saturase, PPAR-alpha-regulated and Starvation-induced Gene Protein, PPSIG, UNQ439/PRO872)
Reactivity
Human
Applications
Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
RetSat; Polyclonal Antibody; RetSat (Retinol Saturase; All-trans-retinol 13; 14-reductase; All-trans-13; 14-dihydroretinol Saturase; PPAR-alpha-regulated and Starvation-induced Gene Protein; PPSIG; UNQ439/PRO872); Anti -RetSat (Retinol Saturase; anti-RETSAT antibody
Host
Mouse
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human RetSat.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Sequence
MHALLVNHYMKGGFYPRGGSSQIAFHTIPVIQRAGGAVLTKATVQSVLLDSAGKACGVSVKKGHELVNIYCPIVVSNAGLFNTYEHLLPGNARCLPGVKQQLGTVRPGLGMTSVFICLRGTKEDLHLPSTNYYVYYDTDMDQAMERYVSMPREEAAEHIPLLFFAFPSAKDPTWEDRFPGGECDCRIPTHQPVLSGCSPRCLLRG
Applicable Applications for anti-RETSAT antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Immunogen
Full length human RetSat, aa1-205 (AAH11418).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of RetSat expression in transfected 293T cell line by RetSat polyclonal antibody. Lane 1: RetSat transfected lysate (22.55kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of RetSat expression in transfected 293T cell line by RetSat polyclonal antibody. Lane 1: RetSat transfected lysate (22.55kD). Lane 2: Non-transfected lysate.)
Product Categories/Family for anti-RETSAT antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
66,820 Da
NCBI Official Full Name
RETSAT protein
NCBI Official Synonym Full Names
retinol saturase (all-trans-retinol 13,14-reductase)
NCBI Official Symbol
RETSAT
NCBI Protein Information
all-trans-retinol 13,14-reductase; all-trans-13,14-dihydroretinol saturase; PPAR-alpha-regulated and starvation-induced gene protein
UniProt Protein Name
All-trans-retinol 13,14-reductase
Protein Family
UniProt Gene Name
RETSAT
UniProt Synonym Gene Names
PPSIG; RetSat
UniProt Entry Name
RETST_HUMAN
Uniprot Description
RETSAT: Retinol saturase carrying out the saturation of the 13- 14 double bond of all-trans-retinol to produce all-trans-13,14- dihydroretinol. Has activity toward all-trans-retinol as substrate. Does not use all-trans-retinoic acid nor 9-cis, 11-cis or 13-cis-retinol isomers as substrates. May play a role in the metabolism of vitamin A. Belongs to the carotenoid/retinoid oxidoreductase family. CrtISO subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cofactor and Vitamin Metabolism - retinol; Secreted, signal peptide; EC 1.3.99.23; Oxidoreductase; Secreted
Chromosomal Location of Human Ortholog: 2p11.2
Cellular Component: nuclear outer membrane; endoplasmic reticulum membrane; nuclear membrane; membrane
Molecular Function: all-trans-retinol 13,14-reductase activity; oxidoreductase activity
Biological Process: retinol metabolic process
Research Articles on RETSAT
Similar Products
Product Notes
The RETSAT retsat (Catalog #AAA6012596) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RetSat (Retinol Saturase, All-trans-retinol 13,14-reductase, All-trans-13,14-dihydroretinol Saturase, PPAR-alpha-regulated and Starvation-induced Gene Protein, PPSIG, UNQ439/PRO872) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RetSat can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Researchers should empirically determine the suitability of the RETSAT retsat for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MHALLVNHYM KGGFYPRGGS SQIAFHTIPV IQRAGGAVLT KATVQSVLLD SAGKACGVSV KKGHELVNIY CPIVVSNAGL FNTYEHLLPG NARCLPGVKQ QLGTVRPGLG MTSVFICLRG TKEDLHLPST NYYVYYDTDM DQAMERYVSM PREEAAEHIP LLFFAFPSAK DPTWEDRFPG GECDCRIPTH QPVLSGCSPR CLLRG. It is sometimes possible for the material contained within the vial of "RetSat, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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