Rabbit RBX1/ROC1 Polyclonal Antibody | anti-RBX1/ROC1 antibody

Anti-RBX1/ROC1 Antibody

Cat. Num. AAA2401921

• Gene Names: RBX1; ROC1; RNF75; BA554C12.1
• Reactivity: Human; Predicted: Mouse, Rat
• Applications: Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Western Blot
• Purity: Affinity Purified
• Synonyms: RBX1/ROC1; Polyclonal Antibody; Anti-RBX1/ROC1 Antibody; Rabbit Polyclonal to Human RBX1/ROC1; RBX1; Regulator of cullins 1; RING box protein 1; ROC1; TESTICAN; Ring-box 1; RNF75; BA554C12.1; Protein ZYP; RING finger protein 75; RING-box protein 1; ZYP protein; anti-RBX1/ROC1 antibody

• Host: Rabbit
• Reactivity: Human; Predicted: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human ROC1/RBX1
• Purity/Purification: Affinity Purified
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3
• Sequence Length: 108

• Applicable Applications for anti-RBX1/ROC1 antibody: Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IF: 1:50-1:200; IHC: 1:50-1:200; IHC-P: 1:100; WB: 1:500-1:2000
• Immunogen: Recombinant protein of human RBX1
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunofluorescence (IF)

(Immunofluorescence analysis of MCF7 cells.)

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using RBX1 antibody.)

Western Blot (WB)

(Western blot analysis of extracts of various cell lines.)

Related Product Information for anti-RBX1/ROC1 antibody

ubiquitin ligase component of multiple cullin-RING-based E3ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. the functional specificity of the E3 ubiquitin-protein ligase complexesdepends on the variable substrate recognition components.

NCBI and Uniprot Product Information

• NCBI GI #: 7657508
• NCBI GeneID: 9978
• NCBI Accession #: NP_055063.1
• NCBI GenBank Nucleotide #: NM_014248.3
• UniProt Accession #: P62877; Q8N6Z8; Q9D1S2; Q9WUK9; Q9Y254; B2RDY1
• Molecular Weight: 12,274 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase RBX1
• NCBI Official Synonym Full Names: ring-box 1
• NCBI Official Symbol: RBX1
• NCBI Official Synonym Symbols: ROC1; RNF75; BA554C12.1
• NCBI Protein Information: E3 ubiquitin-protein ligase RBX1
• UniProt Protein Name: E3 ubiquitin-protein ligase RBX1
• UniProt Gene Name: RBX1
• UniProt Synonym Gene Names: RNF75; ROC1; Rbx1

NCBI Description

This locus encodes a RING finger-like domain-containing protein. The encoded protein interacts with cullin proteins and likely plays a role in ubiquitination processes necessary for cell cycle progression. This protein may also affect protein turnover. Related pseudogenes exist on chromosomes 2 and 5.[provided by RefSeq, Sep 2010]

Uniprot Description

E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase (CRLs) complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. CRLs complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins, ARIH1 mediating addition of the first ubiquitin on CRLs targets (PubMed:27565346). The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme, like CDC34, to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M. Involved in the ubiquitination of KEAP1, ENC1 and KLHL41. In concert with ATF2 and CUL3, promotes degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM.