Typical Standard Curve/Testing Data
Rat Y+L amino acid transporter 1 (SLC7A7) ELISA Kit | SLC7A7 elisa kit
Rat Y+L amino acid transporter 1 (SLC7A7) ELISA Kit
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Principle of the Assay: SLC7A7 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-SLC7A7 antibody and an SLC7A7-HRP conjugate. The assay sample and buffer are incubated together with SLC7A7-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SLC7A7 concentration since SLC7A7 from samples and SLC7A7-HRP conjugate compete for the anti-SLC7A7 antibody binding site. Since the number of sites is limited, as more sites are occupied by SLC7A7 from the sample, fewer sites are left to bind SLC7A7-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SLC7A7 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is the light subunit of a cationic amino acid transporter. This sodium-independent transporter is formed when the light subunit encoded by this gene dimerizes with the heavy subunit transporter protein SLC3A2. This transporter is found in epithelial cell membranes where it transfers cationic and large neutral amino acids from the cell to the extracellular space. Defects in this gene are a cause of lysinuric protein intolerance (LPI). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2011]
Uniprot Description
SLC7A7: Involved in the sodium-independent uptake of dibasic amino acids and sodium-dependent uptake of some neutral amino acids. Requires coexpression with SLC3A2/4F2hc to mediate the uptake of arginine, leucine and glutamine. Plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. Involved in the transport of L- arginine in monocytes. Defects in SLC7A7 are the cause of lysinuric protein intolerance (LPI). LPI is an autosomal recessive multisystem disorder found mainly in Finland and Italy. On a normal diet, LPI patients present poor feeding, vomiting, diarrhea, episodes of hyperammoniaemic coma and growth retardation. Hepatosplenomegaly, osteoporosis and a life- threatening pulmonary involvement (alveolar proteinosis) are also seen. Biochemically LPI is characterized by a defect in the plasma membrane transport of dibasic amino acids. Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.
Protein type: Transporter, SLC family; Membrane protein, integral; Transporter; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 14q11.2
Cellular Component: basolateral plasma membrane; integral to plasma membrane; plasma membrane
Molecular Function: amino acid transmembrane transporter activity
Biological Process: amino acid metabolic process; amino acid transport; transport; protein complex assembly; ion transport; blood coagulation; transmembrane transport; leukocyte migration
Disease: Lysinuric Protein Intolerance
Research Articles on SLC7A7
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Product Notes
The Rat SLC7A7 slc7a7 (Catalog #AAA7219710) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7219710 ELISA Kit recognizes Rat SLC7A7. It is sometimes possible for the material contained within the vial of "Y+L amino acid transporter 1 (SLC7A7), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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