Rabbit E2F8 Polyclonal Antibody | anti-E2F8 antibody

E2F8 Antibody (C-Term)

Cat. Num. AAA9216140

• Gene Names: E2F8; E2F-8
• Reactivity: Human; Predicted: Mouse, Rat
• Applications: Western Blot
• Synonyms: E2F8; Polyclonal Antibody; E2F8 Antibody (C-Term); Purified Rabbit Polyclonal Antibody (Pab); Transcription factor E2F8; E2F-8; anti-E2F8 antibody

• Host: Rabbit
• Reactivity: Human; Predicted: Mouse, Rat
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This E2F8 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 835-865 amino acids from human E2F8.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 867

• Applicable Applications for anti-E2F8 antibody: Western Blot (WB)
• Application Notes: WB ~~ 1:2000
• Antigen Source: Human
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-E2F8 Antibody (C-Term) at 1:2000 dilutionLane 1: A431 whole cell lysateLane 2: HepG2 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 94 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-E2F8 antibody

Atypical E2F transcription factor that participates in various processes such as angiogenesis and polyploidization of specialized cells. Mainly acts as a transcription repressor that binds DNA independently of DP proteins and specifically recognizes the E2 recognition site 5'-TTTC[CG]CGC-3'. Directly represses transcription of classical E2F transcription factors such as E2F1: component of a feedback loop in S phase by repressing the expression of E2F1, thereby preventing p53/TP53-dependent apoptosis. Plays a key role in polyploidization of cells in placenta and liver by regulating the endocycle, probably by repressing genes promoting cytokinesis and antagonizing action of classical E2F proteins (E2F1, E2F2 and/or E2F3). Required for placental development by promoting polyploidization of trophoblast giant cells. Acts as a promoter of sprouting angiogenesis, possibly by acting as a transcription activator: associates with HIF1A, recognizes and binds the VEGFA promoter, which is different from canonical E2 recognition site, and activates expression of the VEGFA gene.

Product Categories/Family for anti-E2F8 antibody

Cancer; Metabolism

References

Ota T.,et al.Nat. Genet. 36:40-45(2004). Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases. Totoki Y.,et al.Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases. Christensen J.,et al.Nucleic Acids Res. 33:5458-5470(2005). Logan N.,et al.Oncogene 24:5000-5004(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 373838711
• NCBI GeneID: 79733
• NCBI Accession #: NP_001243300.1
• NCBI GenBank Nucleotide #: NM_001256371.1
• UniProt Accession #: A0AVK6; Q2VPJ3; Q3C1U6; Q5BKY4; Q8N340; Q9H5M0; A8K9H3
• Molecular Weight: 94166
• NCBI Official Full Name: transcription factor E2F8
• NCBI Official Synonym Full Names: E2F transcription factor 8
• NCBI Official Symbol: E2F8
• NCBI Official Synonym Symbols: E2F-8
• NCBI Protein Information: transcription factor E2F8
• UniProt Protein Name: Transcription factor E2F8
• Protein Family: Transcription factor
• UniProt Gene Name: E2F8
• UniProt Synonym Gene Names: E2F-8
• UniProt Entry Name: E2F8_HUMAN

NCBI Description

This gene encodes a member of a family of transcription factors which regulate the expression of genes required for progression through the cell cycle. The encoded protein regulates progression from G1 to S phase by ensuring the nucleus divides at the proper time. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jan 2012]

Uniprot Description

E2F8: Along with E2F7, inhibitor of E2F-dependent transcription. Binds DNA independently of DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Directly represses E2F1 transcription. Appears to regulate a subset of E2F-dependent genes whose products are required for normal cell cycle progession. Belongs to the E2F/DP family.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: transcription factor complex; nucleus

Molecular Function: protein binding; protein homodimerization activity; transcription corepressor activity; transcription factor activity

Biological Process: cell proliferation; transcription, DNA-dependent; positive regulation of DNA endoreduplication; positive regulation of transcription from RNA polymerase II promoter; sprouting angiogenesis; cell cycle comprising mitosis without cytokinesis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of cytokinesis; placenta development