Rabbit anti-Human, Mouse E2F6 Polyclonal Antibody | anti-E2F6 antibody

E2F6 Antibody

Cat. Num. AAA9413470

• Gene Names: E2F6; E2F-6
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: E2F6; Polyclonal Antibody; E2F6 Antibody; E2F-6; E2F transcription factor 6 isoform 1; E2F transcription factor 6 isoform 2; E2F transcription factor 6 isoform 3; E2F transcription factor 6 isoform 4; anti-E2F6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total E2F6 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 249

• Applicable Applications for anti-E2F6 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500~1:3000; Immunohistochemistry: 1:50~1:100
• Immunogen Type: Peptide
• Immunogen Description: Synthesized peptide derived from human E2F6.
• Target Name: E2F6
• Preparation and Storage: Store at -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human brain tissue using E2F6 antibody.)

Western Blot (WB)

(Western blot analysis of extracts from HepG2 cells, using E2F6 antibody.)

Western Blot (WB)

(Western blot analysis of extracts from colo cells (Lane 2), using E2F6 antiobdy. The lane on the left is treated with synthesized peptide.)

Related Product Information for anti-E2F6 antibody

Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase.Matthew J. Oberley, J. Biol. Chem., Oct 2003; 278: 42466 - 42476.Claire Attwooll, J. Biol. Chem., Jan 2005; 280: 1199 - 1208.Paloma H. Giangrande, Genes & Dev., Dec 2004; 18: 2941 - 2951.J rg Storre, J. Biol. Chem., Dec 2005; 280: 41380 - 41386.

Product Categories/Family for anti-E2F6 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 506949344
• NCBI GeneID: 1876
• NCBI Accession #: NP_001265204.1
• NCBI GenBank Nucleotide #: NM_001278275.1
• UniProt Accession #: O75461; O60544; Q53QY9; Q6Q9Z6; Q7Z2H6; A8K2Z8; G5E936
• Molecular Weight: 28,103 Da
• NCBI Official Full Name: transcription factor E2F6 isoform 2
• NCBI Official Synonym Full Names: E2F transcription factor 6
• NCBI Official Symbol: E2F6
• NCBI Official Synonym Symbols: E2F-6
• NCBI Protein Information: transcription factor E2F6
• UniProt Protein Name: Transcription factor E2F6
• Protein Family: Transcription factor
• UniProt Gene Name: E2F6
• UniProt Synonym Gene Names: E2F-6
• UniProt Entry Name: E2F6_HUMAN

NCBI Description

This gene encodes a member of a family of transcription factors that play a crucial role in the control of the cell cycle. The protein encoded by this gene lacks the transactivation and tumor suppressor protein association domains found in other family members, and contains a modular suppression domain that functions in the inhibition of transcription. It interacts in a complex with chromatin modifying factors. There are pseudogenes for this gene on chromosomes 22 and X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]

Uniprot Description

E2F6: Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase. Belongs to the E2F/DP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 2p25.1

Cellular Component: transcription factor complex; nucleus

Molecular Function: DNA binding; transcription factor activity; transcription corepressor activity

Biological Process: G1/S-specific transcription in mitotic cell cycle; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter