Western Blot (WB)
(Anti-E2F6 antibody, MBS176156, Western blottingAll lanes: Anti E2F6 (MBS176156) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: COLO320 Whole Cell Lysate at 40ugLane 3: A549 Whole Cell Lysate at 40ugLane 4: MCF-7 Whole Cell Lysate at 40ugLane 5: SMMC Whole Cell Lysate at 40ugPredicted bind size: 32KDObserved bind size: 32KD)
Rabbit E2F6 Polyclonal Antibody | anti-E2F6 antibody
Anti-E2F6 antibody
Western Blot (WB)
(Anti-E2F6 antibody, MBS176156, Western blottingAll lanes: Anti E2F6 (MBS176156) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: COLO320 Whole Cell Lysate at 40ugLane 3: A549 Whole Cell Lysate at 40ugLane 4: MCF-7 Whole Cell Lysate at 40ugLane 5: SMMC Whole Cell Lysate at 40ugPredicted bind size: 32KDObserved bind size: 32KD)
Western Blot (WB)
(Anti-E2F6 antibody, MBS176156, Western blottingAll lanes: Anti E2F6 (MBS176156) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: COLO320 Whole Cell Lysate at 40ugLane 3: A549 Whole Cell Lysate at 40ugLane 4: MCF-7 Whole Cell Lysate at 40ugLane 5: SMMC Whole Cell Lysate at 40ugPredicted bind size: 32KDObserved bind size: 32KD)
Background: E2F6(E2F transcription factor 6) also known as E2F-6. Northern blot analysis revealed that E2F6 is expressed as 2 mRNAs, 2.5 and 3.5 kb, in all human tissues and cell lines tested. The predicted human and mouse E2F6 protein sequences are 92% identical. The DNA-binding and dimerization domains of E2F6 are highly related to those of other E2F family members, but this protein lacks the sequences necessary for either transcriptional activation or binding to RB1, p107, or p130. E2F6 can act to repress the transcription of E2F-responsive genes by countering the activity of other E2F complexes. E2F6 contributes to gene silencing in a manner independent of retinoblastoma protein family members. E2F6 is found in a multimeric protein complex that contains MGA and MAX, and thus the complex can bind not only to the E2F binding site but also to MYC- and Brachyury- binding sites. The E2F6 complex preferentially occupies target promoters in G0 cells rather than in G1 cells.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of a family of transcription factors that play a crucial role in the control of the cell cycle. The protein encoded by this gene lacks the transactivation and tumor suppressor protein association domains found in other family members, and contains a modular suppression domain that functions in the inhibition of transcription. It interacts in a complex with chromatin modifying factors. There are pseudogenes for this gene on chromosomes 22 and X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]
Uniprot Description
E2F6: Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase. Belongs to the E2F/DP family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 2p25.1
Cellular Component: transcription factor complex; nucleus
Molecular Function: DNA binding; transcription factor activity; transcription corepressor activity
Biological Process: G1/S-specific transcription in mitotic cell cycle; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter
Research Articles on E2F6
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Product Notes
The E2F6 e2f6 (Catalog #AAA176156) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-E2F6 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's E2F6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the E2F6 e2f6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "E2F6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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