Rabbit E2F4 Polyclonal Antibody | anti-E2F4 antibody

E2F4 Antibody

Cat. Num. AAA9504581

• Gene Names: E2F4; E2F-4
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using immunogen.
• Synonyms: E2F4; Polyclonal Antibody; E2F4 Antibody; E2F-4; p107/p130-binding protein; anti-E2F4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: E2F4 Antibody detects endogenous levels of total E2F4 protein.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 413

• Applicable Applications for anti-E2F4 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:40000
• Target Modification: Unmodified/Total
• Immunogen: The antiserum was produced against synthesized peptide derived from human E2F4.
• Immunogen Range: 51-100
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using E2F4 Antibody. The picture on the right is blocked with the synthesized peptide.)

Western Blot (WB)

(Western blot analysis of lysates from COS7 cells, using E2F4 Antibody. The lane on the right is blocked with the synthesized peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 12669915
• NCBI GeneID: 1874
• NCBI Accession #: NP_001941.2
• NCBI GenBank Nucleotide #: NM_001950.3
• UniProt Accession #: Q16254; Q12991; Q15328; A6NGR8; B5BU56
• Molecular Weight: 43 kDa
• NCBI Official Full Name: transcription factor E2F4
• NCBI Official Synonym Full Names: E2F transcription factor 4
• NCBI Official Symbol: E2F4
• NCBI Official Synonym Symbols: E2F-4
• NCBI Protein Information: transcription factor E2F4
• UniProt Protein Name: Transcription factor E2F4
• Protein Family: Transcription factor
• UniProt Gene Name: E2F4
• UniProt Synonym Gene Names: E2F-4

NCBI Description

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer. [provided by RefSeq, Jul 2008]

Uniprot Description

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.