Western Blot (WB)
(Western blot analysis of DYNC1I2 using LOVO whole cell lysates)
Rabbit DYNC1I2 Polyclonal Antibody | anti-DYNC1I2 antibody
DYNC1I2 Antibody
Gene Names
DYNC1I2; IC2; DIC74; DNCI2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
DYNC1I2; Polyclonal Antibody; DYNC1I2 Antibody; Cytoplasmic dynein 1 intermediate chain 2; Cytoplasmic dynein intermediate chain 2; cytosolic; DC1I2_HUMAN; DH IC 2; DH IC-2; DNCI2; DNCIC2; Dync1i2; Dynein intermediate chain 2; Dynein; cytoplasmic; intermediate polypeptide 2; IC2; anti-DYNC1I2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
DYNC1I2 antibody detects endogenous levels of total DYNC1I2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
638
Applicable Applications for anti-DYNC1I2 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IHC: 1:200
IHC: 1:200
Immunogen
A synthesized peptide derived from human DYNC1I2
Subcellular Location
Cytoplasm > Cytoskeleton.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of DYNC1I2 using LOVO whole cell lysates)
Western Blot (WB)
(Western blot analysis of DYNC1I2 using LOVO whole cell lysates)
Immunohistochemistry (IHC)
(MBS9609744 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9609744 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-DYNC1I2 antibody
Function: Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCNT1. Involved in membrane-transport, such as Golgi apparatus, late endosomes and lysosomes.
Subunit Structure: Homodimer (By similarity). The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits presented by intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs); the composition seems to vary in respect to the IC, LIC and LC composition. The heavy chain homodimer serves as a scaffold for the probable homodimeric assembly of the respective non-catalytic subunits. The ICs and LICs bind directly to the HC dimer and the LCs assemble on the IC dimer. Interacts with DYNLT1 and DYNLT3. Interacts with DCNT1 (By similarity). Interacts with human adenovirus 5 hexon protein; this interaction probably allows virus intracellular transport (PubMed:20006841). Interacts with BICD2 (By similarity).
Post-translational Modifications: The phosphorylation status of Ser-90 appears to be involved in dynactin-dependent target binding.
Similarity: Belongs to the dynein intermediate chain family.
Subunit Structure: Homodimer (By similarity). The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits presented by intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs); the composition seems to vary in respect to the IC, LIC and LC composition. The heavy chain homodimer serves as a scaffold for the probable homodimeric assembly of the respective non-catalytic subunits. The ICs and LICs bind directly to the HC dimer and the LCs assemble on the IC dimer. Interacts with DYNLT1 and DYNLT3. Interacts with DCNT1 (By similarity). Interacts with human adenovirus 5 hexon protein; this interaction probably allows virus intracellular transport (PubMed:20006841). Interacts with BICD2 (By similarity).
Post-translational Modifications: The phosphorylation status of Ser-90 appears to be involved in dynactin-dependent target binding.
Similarity: Belongs to the dynein intermediate chain family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 71 kDa
Predicted: 72 kDa
Predicted: 72 kDa
NCBI Official Full Name
cytoplasmic dynein 1 intermediate chain 2 isoform 1
NCBI Official Synonym Full Names
dynein cytoplasmic 1 intermediate chain 2
NCBI Official Symbol
DYNC1I2
NCBI Official Synonym Symbols
IC2; DIC74; DNCI2
NCBI Protein Information
cytoplasmic dynein 1 intermediate chain 2
UniProt Protein Name
Cytoplasmic dynein 1 intermediate chain 2
Protein Family
UniProt Gene Name
DYNC1I2
UniProt Synonym Gene Names
DNCI2; DNCIC2; DH IC-2
NCBI Description
This gene encodes a member of the dynein intermediate chain family. The encoded protein is a non-catalytic component of the cytoplasmic dynein 1 complex, which acts as a retrograde microtubule motor to transport organelles and vesicles. A pseudogene of this gene is located on chromosome 10. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]
Uniprot Description
Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCNT1. Involved in membrane-transport, such as Golgi apparatus, late endosomes and lysosomes.
Research Articles on DYNC1I2
Similar Products
Product Notes
The DYNC1I2 dync1i2 (Catalog #AAA9609744) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DYNC1I2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DYNC1I2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:1000-3000 IHC: 1:200. Researchers should empirically determine the suitability of the DYNC1I2 dync1i2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DYNC1I2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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