Western Blot (WB)
((2ug/ml) staining of Human Cerebellum (A), Mouse (B), and Rat (C) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat DUSP8/HVH5 Polyclonal Antibody | anti-DUSP8 antibody
Goat anti-DUSP8/HVH5 Antibody
Western Blot: Approx 55-58kDa band observed in Human (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 65.8kDa according to Human NP_004411.2, 68.8kDa according to Mouse NP_032774.1 and 66.8kDa according to Rat NP_001101980.1). Recommended concentration: 1-3ug/ml.
Western Blot (WB)
((2ug/ml) staining of Human Cerebellum (A), Mouse (B), and Rat (C) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((2ug/ml) staining of Human Cerebellum (A), Mouse (B), and Rat (C) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates SAPK/JNK and p38, is expressed predominantly in the adult brain, heart, and skeletal muscle, is localized in the cytoplasm, and is induced by nerve growth factor and insulin. An intronless pseudogene for DUSP8 is present on chromosome 10q11.2. [provided by RefSeq, Jul 2008]
Uniprot Description
DUSP8: a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (ERK, JNK, p38). Inactivates SAPK/JNK and p38, is expressed predominantly in the adult brain, heart, and skeletal muscle, is localized in the cytoplasm, and is induced by nerve growth factor and insulin.
Protein type: Protein phosphatase, dual-specificity; EC 3.1.3.16; EC 3.1.3.48; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 11p15.5
Cellular Component: cytoplasm; nucleus
Molecular Function: MAP kinase tyrosine/serine/threonine phosphatase activity; protein tyrosine phosphatase activity
Biological Process: inactivation of MAPK activity; protein amino acid dephosphorylation
Research Articles on DUSP8
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Product Notes
The DUSP8 dusp8 (Catalog #AAA420217) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-DUSP8/HVH5 Antibody reacts with Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's DUSP8/HVH5 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 4000. Western Blot: Approx 55-58kDa band observed in Human (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 65.8kDa according to Human NP_004411.2, 68.8kDa according to Mouse NP_032774.1 and 66.8kDa according to Rat NP_001101980.1). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the DUSP8 dusp8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: AGDRLPRKVM DAK. It is sometimes possible for the material contained within the vial of "DUSP8/HVH5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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