Rabbit anti-Human DUSP6 Polyclonal Antibody | anti-DUSP6 antibody

DUSP6 (Dual Specificity Protein Phosphatase 6, Dual Specificity Protein Phosphatase PYST1, Mitogen-activated Protein Kinase Phosphatase 3, MAP Kinase Phosphatase 3, MKP3, MKP-3, PYST1) (PE)

Cat. Num. AAA6473176

• Gene Names: DUSP6; HH19; MKP3; PYST1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: DUSP6; Polyclonal Antibody; DUSP6 (Dual Specificity Protein Phosphatase 6; Dual Specificity Protein Phosphatase PYST1; Mitogen-activated Protein Kinase Phosphatase 3; MAP Kinase Phosphatase 3; MKP3; MKP-3; PYST1) (PE); Dual Specificity Phosphatase 6; EC=3.1.3.16; EC=3.1.3.48; anti-DUSP6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DUSP6.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 235

• Applicable Applications for anti-DUSP6 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the central region of human DUSP6 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of DUSP6 Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Western Blot (WB)

(The anti-DUSP6 Pab is used in Western blot to detect DUSP6 in HeLa cell lysate.)

Product Categories/Family for anti-DUSP6 antibody

Antibodies; Enzymes; Phosphatase

References

1. Mod. Pathol. 18(8), 1034-1042 (2005). 2. J. Hum. Genet. 50(4), 159-167 (2005). 3. J. Biol. Chem. 279(40), 41882-41891 (2004). 4. Biochemistry 42(51), 15197-15207 (2003). 5. J. Biol. Chem. 271(8), 4319-4326 (1996).

NCBI and Uniprot Product Information

• NCBI GI #: 42764687
• NCBI GeneID: 1848
• NCBI Accession #: NP_073143; NP_001937
• NCBI GenBank Nucleotide #: NP_073143.2
• UniProt Accession #: Q16828
• Molecular Weight: 42.334
• NCBI Official Full Name: dual specificity protein phosphatase 6 isoform b
• NCBI Official Synonym Full Names: dual specificity phosphatase 6
• NCBI Official Symbol: DUSP6
• NCBI Official Synonym Symbols: HH19; MKP3; PYST1
• NCBI Protein Information: dual specificity protein phosphatase 6
• UniProt Protein Name: Dual specificity protein phosphatase 6
• Protein Family: Dual specificity protein phosphatase
• UniProt Gene Name: DUSP6
• UniProt Synonym Gene Names: MKP3; PYST1; MAP kinase phosphatase 3; MKP-3
• UniProt Entry Name: DUS6_HUMAN

NCBI Description

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm. Mutations in this gene have been associated with congenital hypogonadotropic hypogonadism. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2014]

Uniprot Description

MKP-3: a non-receptor, dual-specificity phosphoprotein phosphatase. Different members of the DUSP family show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This protein inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm. Two alternatively spliced isoforms have been described.

Protein type: Protein phosphatase, dual-specificity; Motility/polarity/chemotaxis; EC 3.1.3.48; EC 3.1.3.16

Chromosomal Location of Human Ortholog: 12q22-q23

Cellular Component: nucleoplasm; cytoplasm; cytosol

Molecular Function: protein tyrosine phosphatase activity; MAP kinase tyrosine/serine/threonine phosphatase activity

Biological Process: response to drug; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; regulation of fibroblast growth factor receptor signaling pathway; toll-like receptor 3 signaling pathway; response to organic cyclic substance; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; response to nitrosative stress; dorsal/ventral pattern formation; toll-like receptor signaling pathway; regulation of endodermal cell fate specification; innate immune response; toll-like receptor 9 signaling pathway; cell differentiation; toll-like receptor 4 signaling pathway; inactivation of MAPK activity

Disease: Hypogonadotropic Hypogonadism 19 With Or Without Anosmia