Rabbit anti-Human DUSP6 Polyclonal Antibody | anti-DUSP6 antibody

DUSP6 Antibody

Cat. Num. AAA9411768

• Gene Names: DUSP6; HH19; MKP3; PYST1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Synonyms: DUSP6; Polyclonal Antibody; DUSP6 Antibody; dual specificity phosphatase 6; MKP3; PYST1; anti-DUSP6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total DUSP6 protein.
• Form/Format: Supplied at 0.9mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.
• Sequence Length: 2212

• Applicable Applications for anti-DUSP6 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: ELISA: 1:1000-1:5000; Western blotting: 1:500-1:2000; Immunohistochemistry: 1:25-1:100
• Immunogen Type: Recombinant protein
• Immunogen Description: Fusion protein corresponding to a region derived from 206-381 amino acids of human dual specificity phosphatase 6
• Target Name: DUSP6
• Preparation and Storage: Store at -20 degree C/1 year

Testing Data

(Gel: 10%SDS-PAGELane1: Jurkat cell lysateLane2: Human fetal kidney tissue lysateLysates: 30ug per lanePrimary antibody: 1/450 dilutionSecondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilutionExposure time: 1 minute)

Immunohistochemistry (IHC)

(The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 31069(DUSP6 Antibody) at dilution 1/25, on the right is treated with the fusion protein.)

Related Product Information for anti-DUSP6 antibody

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-DUSP6 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 13477170
• NCBI GeneID: 1848
• Molecular Weight: 26,468 Da
• NCBI Official Full Name: Homo sapiens dual specificity phosphatase 6, mRNA
• NCBI Official Synonym Full Names: dual specificity phosphatase 6
• NCBI Official Symbol: DUSP6
• NCBI Official Synonym Symbols: HH19; MKP3; PYST1
• NCBI Protein Information: dual specificity protein phosphatase 6
• UniProt Protein Name: Dual specificity protein phosphatase 6
• Protein Family: Dual specificity protein phosphatase
• UniProt Gene Name: DUSP6
• UniProt Synonym Gene Names: MKP3; PYST1; MAP kinase phosphatase 3; MKP-3
• UniProt Entry Name: DUS6_HUMAN

NCBI Description

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm. Mutations in this gene have been associated with congenital hypogonadotropic hypogonadism. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2014]

Uniprot Description

MKP-3: a non-receptor, dual-specificity phosphoprotein phosphatase. Different members of the DUSP family show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This protein inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm. Two alternatively spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; EC 3.1.3.16; EC 3.1.3.48; Protein phosphatase, dual-specificity

Chromosomal Location of Human Ortholog: 12q22-q23

Cellular Component: cytoplasm; cytosol; nucleoplasm

Molecular Function: MAP kinase tyrosine/serine/threonine phosphatase activity; protein tyrosine phosphatase activity

Biological Process: activation of MAPK activity; activation of MAPKK activity; axon guidance; dorsal/ventral pattern formation; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; inactivation of MAPK activity; innate immune response; insulin receptor signaling pathway; MAPKKK cascade; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; Ras protein signal transduction; regulation of endodermal cell fate specification; regulation of fibroblast growth factor receptor signaling pathway; response to drug; response to nitrosative stress; response to organic cyclic substance; small GTPase mediated signal transduction; stress-activated MAPK cascade; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway

Disease: Hypogonadotropic Hypogonadism 19 With Or Without Anosmia