Rabbit DUSP3 Polyclonal Antibody | anti-DUSP3 antibody

Anti-DUSP3 Antibody

Cat. Num. AAA1753132

• Gene Names: DUSP3; VHR
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: DUSP3; Polyclonal Antibody; Anti-DUSP3 Antibody; Dual specificity protein phosphatase 3; Dual specificity protein phosphatase VHR; Vaccinia H1-related phosphatase; VHR; anti-DUSP3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 185

• Applicable Applications for anti-DUSP3 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Direct ELISA (EIA)
• Application Notes: WB: 0.25-0.5ug/ml (Human, Mouse, Rat); ICC: 2ug/ml (Human); IF: 2ug/ml (Human); FC/FACS: 1-3ug/1x106 cells (Human); Direct ELISA: 0.1-0.5ug/ml (Human); Tested Species: In-house tested species with positive results.
• Immunogen: E Coli-derived human DUSP3 recombinant protein (Position: S4-P185).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Flow Cytometry (FC/FACS)

Western Blot (WB)

(Figure 1. Western blot analysis of DUSP3 using anti-DUSP3 antibody.)

Related Product Information for anti-DUSP3 antibody

Description: Rabbit IgG polyclonal antibody for DUSP3 detection. Tested with WB, ICC/IF, FCM, Direct ELISA in Human; Mouse; Rat.

Background: Dual specificity protein phosphatase 3 is an enzyme that in humans is encoded by the DUSP3 gene. It is mapped to 17q21.31. The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene maps in a region that contains the BRCA1 locus which confers susceptibility to breast and ovarian cancer. Although DUSP3 is expressed in both breast and ovarian tissues, mutation screening in breast cancer pedigrees and in sporadic tumors was negative, leading to the conclusion that this gene is not BRCA1.

References

1. Alonso, A., Rahmouni, S., Williams, S., van Stipdonk, M., Jaroszewski, L., Godzik, A., Abraham, R. T., Schoenberger, S. P., Mustelin, T. Tyrosine phosphorylation of VHR phosphatase by ZAP-70. Nature Immun. 4: 44-48, 2003.
2. Ishibashi, T., Bottaro, D. P., Chan, A., Miki, T., Aaronson, S. A. Expression cloning of a human dual-specificity phosphatase. Proc. Nat. Acad. Sci. 89: 12170-12174, 1992.
3. Jones, K. A., Black, D. M., Brown, M. A., Griffiths, B. L., Nicolai, H. M., Chambers, J. A., Bonjardim, M., Xu, C.-F., Boyd, M., McFarlane, R., Korn, B., Poustka, A., North, M. A., Schalkwyk, L., Lehrach, H., Solomon, E. The detailed characterisation of a 400 kb cosmid walk in the BRCA1 region: identification and localisation of 10 genes including a dual-specificity phosphatase. Hum. Molec. Genet. 3: 1927-1934, 1994.

NCBI and Uniprot Product Information

• NCBI GI #: 4758208
• NCBI GeneID: 1845
• NCBI Accession #: NP_004081.1
• NCBI GenBank Nucleotide #: NP_004081.1
• UniProt Accession #: P51452
• NCBI Official Full Name: dual specificity protein phosphatase 3
• NCBI Official Synonym Full Names: dual specificity phosphatase 3
• NCBI Official Symbol: DUSP3
• NCBI Official Synonym Symbols: VHR
• NCBI Protein Information: dual specificity protein phosphatase 3
• UniProt Protein Name: Dual specificity protein phosphatase 3
• Protein Family: Dual specificity protein phosphatase
• UniProt Gene Name: DUSP3
• UniProt Synonym Gene Names: VHR; VHR
• UniProt Entry Name: DUS3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene maps in a region that contains the BRCA1 locus which confers susceptibility to breast and ovarian cancer. Although DUSP3 is expressed in both breast and ovarian tissues, mutation screening in breast cancer pedigrees and in sporadic tumors was negative, leading to the conclusion that this gene is not BRCA1. [provided by RefSeq, Jul 2008]

Uniprot Description

DUSP3: a non-receptor, dual-specificity phosphoprotein phosphatase (DUSP). Different members of the DUSP family show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP3 inactivates the mitogen-activated kinases Erk2 and Jnk and is phosphorylated by ZAP-70. Phosphorylation by ZAP-70 is required for DUSP3 to inhibit the Erk2-Elk-1 pathway.

Protein type: EC 3.1.3.48; Motility/polarity/chemotaxis; Protein phosphatase, dual-specificity; EC 3.1.3.16

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: nucleoplasm; cytoplasm; immunological synapse; cytosol; nucleus

Molecular Function: MAP kinase phosphatase activity; protein tyrosine/serine/threonine phosphatase activity; protein tyrosine phosphatase activity

Biological Process: negative regulation of JNK cascade; nerve growth factor receptor signaling pathway; in utero embryonic development; negative regulation of MAPKKK cascade; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; negative regulation of T cell receptor signaling pathway; toll-like receptor 3 signaling pathway; positive regulation of mitotic cell cycle; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway; negative regulation of T cell activation; inactivation of MAPK activity