Rabbit anti-Human DUSP13 Polyclonal Antibody | anti-DUSP13 antibody

DUSP13, NT (M1) (Dual Specificity Protein Phosphatase 13, Testis-and Skeletal-muscle-specific DSP, Dual Specificity Phosphatase SKRP4, TMDP) (AP)

Cat. Num. AAA6473221

• Gene Names: DUSP13; BEDP; MDSP; TMDP; SKRP4; DUSP13A; DUSP13B
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: DUSP13; Polyclonal Antibody; NT (M1) (Dual Specificity Protein Phosphatase 13; Testis-and Skeletal-muscle-specific DSP; Dual Specificity Phosphatase SKRP4; TMDP) (AP); anti-DUSP13 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DUSP13-M1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-DUSP13 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human DUSP13-M1 protein (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of DUSP13 (arrow) using DUSP13-M1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DUSP13 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-DUSP13 antibody

Antibodies; Enzymes; Phosphatase

References

1. Nakamura K., Shima H., Watanabe M., Haneji T., Kikuchi K.Biochem. J. 344:819-825(1999). 2. Deloukas et al. Nature 429:375-381(2004). 3. Strausberg et al. Proc. Natl. Acad. Sci. USA 99:16899-16903(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 56237018
• NCBI GeneID: 51207
• NCBI Accession #: NP_057448.3
• NCBI GenBank Nucleotide #: NM_016364.3
• UniProt Accession #: Q9UII6; Q5JSC6; Q6IAR0; Q96GC2; A8K776; A8K782; B3KPY1; B3KXT0; B4DUK0; Q6B8I1
• Molecular Weight: 20,658 Da
• NCBI Official Full Name: dual specificity protein phosphatase 13 isoform 6
• NCBI Official Synonym Full Names: dual specificity phosphatase 13
• NCBI Official Symbol: DUSP13
• NCBI Official Synonym Symbols: BEDP; MDSP; TMDP; SKRP4; DUSP13A; DUSP13B
• NCBI Protein Information: dual specificity protein phosphatase 13; branching-enzyme interacting DSP; branching-enzyme interacting dual-specificity protein phosphatase; muscle-restricted DSP; testis- and skeletal-muscle-specific DSP
• UniProt Protein Name: Dual specificity protein phosphatase 13 isoform B
• Protein Family: Dual specificity protein phosphatase
• UniProt Gene Name: DUSP13
• UniProt Synonym Gene Names: DUSP13B; TMDP; DUSP13B
• UniProt Entry Name: DS13B_HUMAN

NCBI Description

Members of the protein-tyrosine phosphatase superfamily cooperate with protein kinases to regulate cell proliferation and differentiation. This superfamily is separated into two families based on the substrate that is dephosphorylated. One family, the dual specificity phosphatases (DSPs) acts on both phosphotyrosine and phosphoserine/threonine residues. This gene encodes different but related DSP proteins through the use of non-overlapping open reading frames, alternate splicing, and presumed different transcription promoters. Expression of the distinct proteins from this gene has been found to be tissue specific and the proteins may be involved in postnatal development of specific tissues. A protein encoded by the upstream ORF was found in skeletal muscle, whereas the encoded protein from the downstream ORF was found only in testis. In mouse, a similar pattern of expression was found. Multiple alternatively spliced transcript variants were described, but the full-length sequence of only some were determined. [provided by RefSeq, Jul 2008]

Uniprot Description

DUSP13: May be involved in the regulation of meiosis and/or differentiation of testicular germ cells during spermatogenesis. Exhibits intrinsic phosphatase activity towards both phospho- seryl/threonyl and -tyrosyl residues of myelin basic protein, with similar specific activities in vitro. Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily. 5 isoforms of the human protein are produced by alternative promoter.

Protein type: Motility/polarity/chemotaxis; Cell cycle regulation; Protein phosphatase, dual-specificity; EC 3.1.3.16; EC 3.1.3.48

Chromosomal Location of Human Ortholog: 10q22.2

Cellular Component: cytoplasm

Molecular Function: protein tyrosine/serine/threonine phosphatase activity; protein tyrosine phosphatase activity

Biological Process: meiosis; spermatogenesis; protein amino acid dephosphorylation