CD3 epsilon/CD3e Recombinant Protein | CD3E recombinant protein

CD3 epsilon/CD3e Protein, Mouse, Recombinant (ECD, hFc Tag)

Cat. Num. AAA8122727

• Gene Names: Cd3e; CD3; T3e; AI504783; CD3epsilon
• Purity: >95% as determined by SDS-PAGE
• Synonyms: CD3 epsilon/CD3e; CD3 epsilon/CD3e Protein; Mouse; Recombinant (ECD; hFc Tag); Mouse CD3e/CD3 epsilon Protein (Fc Tag); AI504783 Protein; CD3 Protein; CD3epsilon Protein; T3e Protein; CD3e molecule; epsilon (CD3-TCR complex); CD3E recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asp108

• Species: Mouse
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Asp 23
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the mouse CD3E (NP_031674.1)(Met1-Asp108) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CD3E recombinant protein

Background: T-cell surface glycoprotein CD3 epsilon chain, also known as CD3E, is a single-pass type I membrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The CD3 epsilon subunit of the T cell receptor (TCR) complex contains two defined signaling domains, a proline-rich sequence and an immune tyrosine activation motifs (ITAMs), and this complex undergoes a conformational change upon ligand binding that is thought to be important for the activation of T cells. In the CD3 epsilon mutant mice, all stages of T cell development and activation that are TCR-dependent were impaired, but not eliminated, including activation of mature naa¯ve T cells with the MHCII presented superantigen, staphylococcal enterotoxin B, or with a strong TCR cross-linking antibody specific for either TCR-Cbeta or CD3 epsilon. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. Homozygous mutations in CD3D and CD3E genes lead to a complete block in T-cell development and thus to an early-onset severe combined immunodeficiency phenotype.

Product Categories/Family for CD3E recombinant protein

ITIM/ITAM Immunoreceptors and Related Molecules; T Cell Receptor Signaling Pathway

References

Fischer A, et al. (2005) CD3 deficiencies. Curr Opin Allergy Clin Immunol. 5(6): 491-5.
Wang Y, et al. (2009) A conserved CXXC motif in CD3epsilon is critical for T cell development and TCR signaling. PLoS Biol. 7(12): e1000253.
Martnez-Martn N, et al. (2009) Cooperativity between T cell receptor complexes revealed by conformational mutants of CD3epsilon. Sci Signal. 2(83): ra43.
Deford-Watts LM, et al. (2009) The cytoplasmic tail of the T cell receptor CD3 epsilon subunit contains a phospholipid-binding motif that regulates T cell functions. J Immunol. 183(2): 1055-64.

NCBI and Uniprot Product Information

• NCBI GI #: 6680886
• NCBI GeneID: 12501
• NCBI Accession #: NP_031674.1
• NCBI GenBank Nucleotide #: NM_007648.4
• Molecular Weight: 21,393 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD3 epsilon chain
• NCBI Official Synonym Full Names: CD3 antigen, epsilon polypeptide
• NCBI Official Symbol: Cd3e
• NCBI Official Synonym Symbols: CD3; T3e; AI504783; CD3epsilon
• NCBI Protein Information: T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain
• Protein Family: T-cell surface glycoprotein