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Western Blot (WB) (Western blot analysis of DUS2L expression in HCT116 (A), mouse lung (B), rat lung (C) whole cell lysates.)

Rabbit DUS2L Polyclonal Antibody | anti-DUS2L antibody

Anti-DUS2L Antibody

Gene Names
DUS2; SMM1; DUS2L; URLC8
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry
Purity
Purified by immunogen affinity chromatography.
Synonyms
DUS2L; Polyclonal Antibody; Anti-DUS2L Antibody; tRNA-dihydrouridine(20) synthase [NAD(P)+]-like; Dihydrouridine synthase 2; Up-regulated in lung cancer protein 8; URLC8; tRNA-dihydrouridine synthase 2-like; hDUS2; anti-DUS2L antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of DUS2L protein.
Purity/Purification
Purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
Sequence Length
493
Applicable Applications for anti-DUS2L antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:100
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human DUS2L.
Preparation and Storage
Upon delivery aliquot and store at-20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of DUS2L expression in HCT116 (A), mouse lung (B), rat lung (C) whole cell lysates.)

Western Blot (WB) (Western blot analysis of DUS2L expression in HCT116 (A), mouse lung (B), rat lung (C) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of DUS2L staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of DUS2L staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Related Product Information for anti-DUS2L antibody
Rabbit polyclonal antibody to DUS2L

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
55,050 Da
NCBI Official Full Name
tRNA-dihydrouridine(20) synthase
NCBI Official Synonym Full Names
dihydrouridine synthase 2
NCBI Official Symbol
DUS2
NCBI Official Synonym Symbols
SMM1; DUS2L; URLC8
NCBI Protein Information
tRNA-dihydrouridine(20) synthase [NAD(P)+]-like
UniProt Protein Name
tRNA-dihydrouridine(20) synthase [NAD(P)+]-like
UniProt Gene Name
DUS2
UniProt Synonym Gene Names
DUS2L; URLC8; hDUS2

NCBI Description

This gene encodes a cytoplasmic protein that catalyzes the conversion of uridine residues to dihydrouridine in the D-loop of tRNA. The resulting modified bases confer enhanced regional flexibility to tRNA. The encoded protein may increase the rate of translation by inhibiting an interferon-induced protein kinase. This gene has been implicated in pulmonary carcinogenesis. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Nov 2012]

Uniprot Description

Dihydrouridine synthase. Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Negatively regulates the activation of EIF2AK2/PKR.

Research Articles on DUS2L

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Product Notes

The DUS2L dus2 (Catalog #AAA8303914) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-DUS2L Antibody reacts with Human, Mouse, Rat, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's DUS2L can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:100. Researchers should empirically determine the suitability of the DUS2L dus2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DUS2L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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