Goat DSS1/SHFM1 Polyclonal Antibody | anti-SHFM1 antibody
Goat anti-DSS1/SHFM1 Antibody
Gene Names
SHFM1; ECD; DSS1; SEM1; SHFD1; SHSF1; Shfdg1
Reactivity
Expected from sequence similarity: Human, Mouse, Dog, Cow, Pig
Applications
ELISA, Immunocytochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
DSS1/SHFM1; Polyclonal Antibody; Goat anti-DSS1/SHFM1 Antibody; SHFM1; split hand/foot malformation (ectrodactyly) type 1; DSS1; Deleted in split-hand/split-foot 1 region; ECD; SEM1; SHFD1; SHSF1; Shfdg1; candidate for split hand/foot malformation type 1; deleted in split-hand/foot 1; deleted in split-hand/split-foot; SHFM1 antibody; split hand/foot malformation (ectrodactyly) type 1 antibody; DSS1 antibody; Deleted in split-hand/split-foot 1 region antibody; ECD antibody; SEM1 antibody; SHFD1 antibody; SHSF1 antibody; Shfdg1 antibody; candidate for split hand/foot malformation type 1 antibody; deleted in split-hand/foot 1 antibody; deleted in split-hand/split-foot 1 antibody; split hand/foot malformation type 1 antibody; DSS1 / SHFM1; anti-SHFM1 antibody
Host
Goat
Reactivity
Expected from sequence similarity: Human, Mouse, Dog, Cow, Pig
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
RAELEKHGYKMETS
Sequence Length
70
Applicable Applications for anti-SHFM1 antibody
Peptide ELISA (EIA), Immunocytochemistry (ICC)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: No signal obtained yet but a preliminary experiment showed low background in Human Liver extract at up to 1ug/ml. This used our standard western blotting protocol which we would not expect to detect proteins as small as the predicted size of approx.8KDa according to NP_006295.
Immunocytochemistry: Anonymous customer found nuclear staining in HT1080.
Western Blot: No signal obtained yet but a preliminary experiment showed low background in Human Liver extract at up to 1ug/ml. This used our standard western blotting protocol which we would not expect to detect proteins as small as the predicted size of approx.8KDa according to NP_006295.
Immunocytochemistry: Anonymous customer found nuclear staining in HT1080.
Immunogen
Peptide with sequence C-RAELEKHGYKMETS, from the C Terminus of the protein sequence according to NP_006295.1.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
8,278 Da
NCBI Official Full Name
26S proteasome complex subunit DSS1
NCBI Official Synonym Full Names
split hand/foot malformation (ectrodactyly) type 1
NCBI Official Symbol
SHFM1
NCBI Official Synonym Symbols
ECD; DSS1; SEM1; SHFD1; SHSF1; Shfdg1
NCBI Protein Information
26S proteasome complex subunit DSS1
UniProt Protein Name
26S proteasome complex subunit DSS1
Protein Family
UniProt Gene Name
SHFM1
UniProt Synonym Gene Names
DSS1; SHFDG1
UniProt Entry Name
DSS1_HUMAN
NCBI Description
The product of this gene has been localized within the split hand/split foot malformation locus SHFM1 at chromosome 7. It has been proposed to be a candidate gene for the autosomal dominant form of the heterogeneous limb developmental disorder split hand/split foot malformation type 1. In addition, it has been shown to directly interact with BRCA2. It also may play a role in the completion of the cell cycle. [provided by RefSeq, Jul 2008]
Uniprot Description
SHFM1: Subunit of the 26S proteasome which plays a role in ubiquitin-dependent proteolysis. Belongs to the DSS1/SEM1 family.
Protein type: DNA repair, damage; Protease; Proteasome complex
Chromosomal Location of Human Ortholog: 7q21.3
Cellular Component: integrator complex; proteasome complex
Molecular Function: protein binding
Biological Process: double-strand break repair via homologous recombination; mRNA export from nucleus; proteasome assembly; proteolysis
Disease: Split-hand/foot Malformation 1
Research Articles on SHFM1
Similar Products
Product Notes
The SHFM1 shfm1 (Catalog #AAA420430) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-DSS1/SHFM1 Antibody reacts with Expected from sequence similarity: Human, Mouse, Dog, Cow, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's DSS1/SHFM1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunocytochemistry (ICC). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: No signal obtained yet but a preliminary experiment showed low background in Human Liver extract at up to 1ug/ml. This used our standard western blotting protocol which we would not expect to detect proteins as small as the predicted size of approx.8KDa according to NP_006295. Immunocytochemistry: Anonymous customer found nuclear staining in HT1080. Researchers should empirically determine the suitability of the SHFM1 shfm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RAELEKHGYK METS. It is sometimes possible for the material contained within the vial of "DSS1/SHFM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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