Rabbit anti-Mouse, Rat DRAM Polyclonal Antibody | anti-DRAM antibody

RABBIT ANTI DRAM (C-TERMINAL)

Cat. Num. AAA224191

• Gene Names: DRAM1; DRAM
• Reactivity: Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Synonyms: DRAM; Polyclonal Antibody; RABBIT ANTI DRAM (C-TERMINAL); anti-DRAM antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 238

• Applicable Applications for anti-DRAM antibody: Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Western Blot: This item detects bands of approximately 16 and 31kDa in 293 cell lysate; Immunohistology - Paraffin: Maximum Dilution: 2.5ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.; Western Blotting: Minimum Dilution: 0.5; Maximum Dilution: 1.0ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide (NaN3)
• Buffer Solution: Phosphate buffered saline
• Antiserum Preparation: Antisera to human DRAM were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blot analysis of whole cell lysate from HEK293 human epithelial kidney cells probed with Rabbit anti Human DRAM (MBS224191) at 0.5(A) and 1(B) ug/ml)

Testing Data #2

(Immunohistochemical staining of human liver with Rabbit anti Human DRAM (MBS224191))

Related Product Information for anti-DRAM antibody

Rabbit anti DRAM (C-terminal) antibody recognizes human DRAM, a lysosomal modulator of autophagy that plays a central role in p53/TP53-mediated apoptosis. DRAM is significantly down-regulated in tumor cell lines by methylation-dependent transcriptional silencing.

NCBI and Uniprot Product Information

• NCBI GI #: 110825978
• NCBI GeneID: 55332
• NCBI Accession #: NP_060840.2
• NCBI GenBank Nucleotide #: NM_018370.2
• UniProt Accession #: Q8N682; Q7L3E3; Q9NUN1; B7Z4T0
• Molecular Weight: 13,972 Da
• NCBI Official Full Name: DNA damage-regulated autophagy modulator protein 1
• NCBI Official Synonym Full Names: DNA-damage regulated autophagy modulator 1
• NCBI Official Symbol: DRAM1
• NCBI Official Synonym Symbols: DRAM
• NCBI Protein Information: DNA damage-regulated autophagy modulator protein 1
• UniProt Protein Name: DNA damage-regulated autophagy modulator protein 1
• UniProt Gene Name: DRAM1
• UniProt Synonym Gene Names: DRAM
• UniProt Entry Name: DRAM1_HUMAN

NCBI Description

This gene is regulated as part of the p53 tumor suppressor pathway. The gene encodes a lysosomal membrane protein that is required for the induction of autophagy by the pathway. Decreased transcriptional expression of this gene is associated with various tumors. This gene has a pseudogene on chromosome 4. [provided by RefSeq, Jul 2008]

Uniprot Description

DRAM1: Lysosomal modulator of autophagy that plays a central role in p53/TP53-mediated apoptosis. Not involved in p73/TP73- mediated autophagy. Belongs to the DRAM/TMEM150 family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Apoptosis

Chromosomal Location of Human Ortholog: 12q23.2

Cellular Component: lysosome; lysosomal membrane; cytoplasm; integral to membrane

Biological Process: apoptosis; autophagy; regulation of autophagy