Rabbit DRAM Polyclonal Antibody | anti-DRAM antibody

DRAM Antibody

Cat. Num. AAA151126

• Gene Names: DRAM1; DRAM
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: DRAM Antibody is affinity chromatography purified via peptide column.
• Synonyms: DRAM; Polyclonal Antibody; DRAM Antibody; DNA damage-regulated autophagy modulator protein 1; Damage-regulated autophagy modulator; damage-regulated autophagy modulator; anti-DRAM antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: DRAM Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 238

• Applicable Applications for anti-DRAM antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: DRAM antibody can be used for detection of DRAM by Western blot at 0.5 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: DRAM antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human DRAM.
• Buffer: DRAM Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: DRAM antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of DRAM in 293 cell lysate with DRAM antibody at (A) 1 and (B) 2 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of DRAM in human liver tissue with DRAM antibody at 2.5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of DRAM in Human Liver tissue with DRAM antibody at 20 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of DRAM in mouse liver tissue with DRAM antibody at 20 μg/mL.Red: DRAM Antibody (4035)Blue: DAPI staining)

Immunohistochemistry (IHC)

(Immunohistochemistry of DRAM in mouse liver tissue with DRAM antibody at 2 μg/mL.)

Related Product Information for anti-DRAM antibody

DRAM Antibody: Damage-regulated autophagy modulator (DRAM) is a p53 target gene encoding a lysosomal protein that induces autophagy, a process that degrades cytosolic proteins and organelles. It has been suggested that activation of DRAM by p53 is simultaneous to the activation by p53 of one or more proapoptotic genes such as PUMA, Bax, etc., and that the signaling pathways regulated by these genes together promote a full cell death response. By itself, DRAM cannot induce apoptosis, but the fact that it is inactivated in certain cancers highlights the importance of DRAM and suggests that autophagy may play a more important role in cancer than initially suspected. At least two different isoforms of DRAM are known to exist.

NCBI and Uniprot Product Information

• NCBI GI #: 22450862
• NCBI GeneID: 55332
• UniProt Accession #: Q8N682; Q7L3E3; Q9NUN1; B7Z4T0
• Molecular Weight: 13,972 Da
• NCBI Official Full Name: Damage-regulated autophagy modulator
• NCBI Official Synonym Full Names: DNA-damage regulated autophagy modulator 1
• NCBI Official Symbol: DRAM1
• NCBI Official Synonym Symbols: DRAM
• NCBI Protein Information: DNA damage-regulated autophagy modulator protein 1
• UniProt Protein Name: DNA damage-regulated autophagy modulator protein 1
• UniProt Gene Name: DRAM1
• UniProt Synonym Gene Names: DRAM
• UniProt Entry Name: DRAM1_HUMAN

NCBI Description

This gene is regulated as part of the p53 tumor suppressor pathway. The gene encodes a lysosomal membrane protein that is required for the induction of autophagy by the pathway. Decreased transcriptional expression of this gene is associated with various tumors. This gene has a pseudogene on chromosome 4. [provided by RefSeq, Jul 2008]

Uniprot Description

DRAM1: Lysosomal modulator of autophagy that plays a central role in p53/TP53-mediated apoptosis. Not involved in p73/TP73- mediated autophagy. Belongs to the DRAM/TMEM150 family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Apoptosis

Chromosomal Location of Human Ortholog: 12q23.2

Cellular Component: lysosomal membrane; lysosome; cytoplasm; integral to membrane

Biological Process: apoptosis; autophagy; regulation of autophagy