Human AQP-1 ELISA Kit | AQP-1 elisa kit

Human AQP-1 (Aquaporin 1) ELISA Kit

Cat. Num. AAA2501188

• Gene Names: Aqp1; CHIP28
• Reactivity: Human
• Synonyms: AQP-1; Human AQP-1 (Aquaporin 1) ELISA Kit; AQP-1 elisa kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant Human AQP-1. No significant cross-reactivity or interference between Human AQP-1 and analogues was observed.
• Sequence Length: 269

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 125-8000pg/mL
• Sensitivity: Min: 75pg/mL; Max: 8000pg/mL
• Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human AQP-1 concentrations in serum, plasma and other biological fluids.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AQP-1 elisa kit

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to AQP-1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for AQP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain AQP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of AQP-1. You can calculate the concentration of AQP-1 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6680710
• NCBI GeneID: 11826
• NCBI Accession #: NP_031498.1
• NCBI GenBank Nucleotide #: NM_007472.2
• UniProt Accession #: Q02013; Q542P1; Q91VY8
• Molecular Weight: 28,793 Da
• NCBI Official Full Name: aquaporin-1
• NCBI Official Synonym Full Names: aquaporin 1
• NCBI Official Symbol: Aqp1
• NCBI Official Synonym Symbols: CHIP28
• NCBI Protein Information: aquaporin-1; AQP-1; DER2; aquaporin-CHIP; delayed early response protein 2; growth factor-induced delayed early response protein; water channel protein for red blood cells and kidney proximal tubule
• UniProt Protein Name: Aquaporin-1
• UniProt Gene Name: Aqp1
• UniProt Synonym Gene Names: AQP-1; DER2
• UniProt Entry Name: AQP1_MOUSE

Uniprot Description

AQP1: Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Belongs to the MIP/aquaporin (TC 1.A.8) family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, aquaporin family

Cellular Component: nuclear membrane; neuron projection; integral to plasma membrane; cell; brush border membrane; basolateral plasma membrane; integral to membrane; dense core granule membrane; membrane; axon; apical part of cell; cytoplasm; apical plasma membrane; basal plasma membrane; plasma membrane; nerve terminal; nucleus; brush border; sarcolemma

Molecular Function: ephrin receptor binding; potassium channel activity; water transporter activity; ammonium transmembrane transporter activity; transporter activity; intracellular cGMP activated cation channel activity; potassium ion transmembrane transporter activity; glycerol transmembrane transporter activity; water channel activity; nitric oxide transporter activity; transmembrane transporter activity; glycerol channel activity

Biological Process: cGMP biosynthetic process; glycerol transport; wound healing; carbon dioxide transport; water transport; cellular response to stress; negative regulation of caspase activity; sensory perception of pain; positive regulation of saliva secretion; water homeostasis; positive regulation of fibroblast proliferation; organic cation transport; nitric oxide transport; transport; cellular homeostasis; transmembrane transport; potassium ion transport; lipid digestion; secretion by cell; response to drug; cellular water homeostasis; secretory granule organization and biogenesis; glomerular filtration; ammonium transport; positive regulation of angiogenesis; renal water transport; camera-type eye morphogenesis; adrenocorticotropin hormone secretion; establishment and/or maintenance of actin cytoskeleton polarity; cell volume homeostasis; negative regulation of apoptosis; positive regulation of cell migration