Western Blot (WB)
(DPYS Antibody (C-term) western blot analysis in Jurkat,K562 cell line lysates (35ug/lane).This demonstrates the DPYS antibody detected the DPYS protein (arrow).)
Rabbit anti-Human DPYS Polyclonal Antibody | anti-DPYS antibody
DPYS, CT (DPYS, Dihydropyrimidinase, Dihydropyrimidine amidohydrolase, Hydantoinase) (FITC)
Gene Names
DPYS; DHP; DHPase
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
DPYS; Polyclonal Antibody; CT (DPYS; Dihydropyrimidinase; Dihydropyrimidine amidohydrolase; Hydantoinase) (FITC); Hydantoinase; anti-DPYS antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-DPYS antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
DPYS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 453-482 amino acids from the C-terminal region of human DPYS.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(DPYS Antibody (C-term) western blot analysis in Jurkat,K562 cell line lysates (35ug/lane).This demonstrates the DPYS antibody detected the DPYS protein (arrow).)
Western Blot (WB)
(DPYS Antibody (C-term) western blot analysis in Jurkat,K562 cell line lysates (35ug/lane).This demonstrates the DPYS antibody detected the DPYS protein (arrow).)
Immunohistochemistry (IHC)
(DPYS Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DPYS Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(DPYS Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DPYS Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-DPYS antibody
Dihydropyrimidinase catalyzes the conversion of 5,6-dihydrouracil to 3-ureidopropionate in pyrimidine metabolism. Dihydropyrimidinase is expressed at a high level in liver and kidney as a major 2.5-kb transcript and a minor 3.8-kb transcript. Defects in the DPYS gene are linked to dihydropyrimidinuria.
Product Categories/Family for anti-DPYS antibody
References
Kim, H.Y., et al. BMB Rep 43(8):547-553(2010). van Kuilenburg, A.B., et al. Biochim. Biophys. Acta 1802 (7-8), 639-648 (2010): Fidlerova, J., et al. Cancer Chemother. Pharmacol. 65(4):661-669(2010). Thomas, H.R., et al. Pharmacogenet. Genomics 18(1):25-35(2008). Thomas, H.R., et al. Pharmacogenet. Genomics 17(11):973-987(2007).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
56,630 Da
NCBI Official Full Name
dihydropyrimidinase
NCBI Official Synonym Full Names
dihydropyrimidinase
NCBI Official Symbol
DPYS
NCBI Official Synonym Symbols
DHP; DHPase
NCBI Protein Information
dihydropyrimidinase
UniProt Protein Name
Dihydropyrimidinase
UniProt Gene Name
DPYS
UniProt Synonym Gene Names
DHP; DHPase
NCBI Description
Dihydropyrimidinase catalyzes the conversion of 5,6-dihydrouracil to 3-ureidopropionate in pyrimidine metabolism. Dihydropyrimidinase is expressed at a high level in liver and kidney as a major 2.5-kb transcript and a minor 3.8-kb transcript. Defects in the DPYS gene are linked to dihydropyrimidinuria. [provided by RefSeq, Jul 2008]
Uniprot Description
Catalyzes the second step of the reductive pyrimidine degradation, the reversible hydrolytic ring opening of dihydropyrimidines. Can catalyze the ring opening of 5,6-dihydrouracil to N-carbamyl-alanine and of 5,6-dihydrothymine to N-carbamyl-amino isobutyrate.
Research Articles on DPYS
Similar Products
Product Notes
The DPYS dpys (Catalog #AAA6293447) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DPYS, CT (DPYS, Dihydropyrimidinase, Dihydropyrimidine amidohydrolase, Hydantoinase) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DPYS can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:10-50 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the DPYS dpys for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DPYS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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