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Western Blot (WB) (Western Blot analysis of 3T3 cells using Moesin/Ezrin/Radixin Polyclonal Antibody diluted at 1?2000)

Rabbit RDX Polyclonal Antibody | anti-RDX antibody

RDX polyclonal antibody

Gene Names
RDX; DFNB24
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
RDX; Polyclonal Antibody; RDX polyclonal antibody; MSN; Moesin; Membrane-organizing extension spike protein; Radixin; EZR; VIL2; Ezrin; Cytovillin; Villin-2; p81; anti-RDX antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Moesin/Ezrin/Radixin Polyclonal Antibody detects endogenous levels of Moesin/Ezrin/Radixin protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Applicable Applications for anti-RDX antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA)
Application Notes
WB: 1/500-1/2000
IHC: 1/100-1/300
ELISA: 1/20,000
Immunogen
The antiserum was produced against synthesized peptide derived from human Moesin/Ezrin/Radixin. AA range: 524-573
Preparation and Storage
Store at 4 degee C for short term. Aliquot and store at -20 degee C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of 3T3 cells using Moesin/Ezrin/Radixin Polyclonal Antibody diluted at 1?2000)

Western Blot (WB) (Western Blot analysis of 3T3 cells using Moesin/Ezrin/Radixin Polyclonal Antibody diluted at 1?2000)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human pancreas tissue, using Moesin/Ezrin/Radixin Antibody. The picture on the right is blocked with the synthesized peptide.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human pancreas tissue, using Moesin/Ezrin/Radixin Antibody. The picture on the right is blocked with the synthesized peptide.)

Western Blot (WB)

(Western blot analysis of lysates from NIH/3T3 cells, using Moesin/Ezrin/Radixin Antibody. The lane on the right is blocked with the synthesized peptide.)

Western Blot (WB) (Western blot analysis of lysates from NIH/3T3 cells, using Moesin/Ezrin/Radixin Antibody. The lane on the right is blocked with the synthesized peptide.)
Related Product Information for anti-RDX antibody
Background: moesin(MSN) Homo sapiens. Moesin (for membrane-organizing extension spike protein) is a member of the ERM family which includes ezrin and radixin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Moesin is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement. [provided by RefSeq, Jul 2008]

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
68,564 Da
NCBI Official Full Name
radixin isoform 3
NCBI Official Synonym Full Names
radixin
NCBI Official Symbol
RDX
NCBI Official Synonym Symbols
DFNB24
NCBI Protein Information
radixin
UniProt Protein Name
Radixin
Protein Family
UniProt Gene Name
RDX
UniProt Entry Name
RADI_HUMAN

NCBI Description

Radixin is a cytoskeletal protein that may be important in linking actin to the plasma membrane. It is highly similar in sequence to both ezrin and moesin. The radixin gene has been localized by fluorescence in situ hybridization to 11q23. A truncated version representing a pseudogene (RDXP2) was assigned to Xp21.3. Another pseudogene that seemed to lack introns (RDXP1) was mapped to 11p by Southern and PCR analyses. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]

Uniprot Description

Radixin: a cytoskeletal protein of the ERM family which includes ezrin and moiesin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. May play a crucial role in the binding of the barbed end of actin filaments to the plasma membrane.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: extracellular space; focal adhesion; T-tubule; ruffle; stereocilium; extrinsic to membrane; cytoskeleton; lamellipodium; apical part of cell; cytoplasm; plasma membrane; filopodium; cleavage furrow

Molecular Function: protein binding; actin binding

Biological Process: microvillus biogenesis; apical protein localization; actin filament capping

Disease: Deafness, Autosomal Recessive 24

Research Articles on RDX

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Product Notes

The RDX rdx (Catalog #AAA3002352) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RDX polyclonal antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RDX can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA). WB: 1/500-1/2000 IHC: 1/100-1/300 ELISA: 1/20,000. Researchers should empirically determine the suitability of the RDX rdx for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RDX, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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