Rabbit anti-Human DPT Polyclonal Antibody | anti-DPT antibody

DPT, ID (Dermatopontin, Tyrosine-rich Acidic Matrix Protein, TRAMP) (Biotin)

Cat. Num. AAA6472286

• Gene Names: DPT; TRAMP
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: DPT; Polyclonal Antibody; ID (Dermatopontin; Tyrosine-rich Acidic Matrix Protein; TRAMP) (Biotin); anti-DPT antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DPT.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-DPT antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human DPT (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of DPT antibody (Center) in HL60 cell line lysates (35ug/lane). DPT (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain with DPT Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of NCI-H292 cells using DPT Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunohistochemistry (IHC)

(Immunofluorescence analysis of DPT Antibody (Center) with paraffin-embedded human brain tissue. 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.)

Product Categories/Family for anti-DPT antibody

Antibodies; Extracellular Matrix Proteins

References

1. Cheung, C.L., Chan, B.Y. Hum. Mol. Genet. 18(4), 679-687 (2009). 2. Pochampally, R.R., Ylostalo, J. J. Bone Miner. Res. 22(9), 1338-1349 (2007). 3. Lunetta, K.L., D'Agostino, R.B. Sr. BMC Med. Genet. 8 SUPPL 1, S13 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 32307172
• NCBI GeneID: 1805
• NCBI Accession #: NP_001928
• NCBI GenBank Nucleotide #: NM_001937
• UniProt Accession #: Q07507
• Molecular Weight: 38
• NCBI Official Full Name: dermatopontin
• NCBI Official Synonym Full Names: dermatopontin
• NCBI Official Symbol: DPT
• NCBI Official Synonym Symbols: TRAMP
• NCBI Protein Information: dermatopontin
• UniProt Protein Name: Dermatopontin
• Protein Family: Dermatopontin
• UniProt Gene Name: DPT
• UniProt Synonym Gene Names: TRAMP

NCBI Description

Dermatopontin is an extracellular matrix protein with possible functions in cell-matrix interactions and matrix assembly. The protein is found in various tissues and many of its tyrosine residues are sulphated. Dermatopontin is postulated to modify the behavior of TGF-beta through interaction with decorin. [provided by RefSeq, Jul 2008]

Uniprot Description

Seems to mediate adhesion by cell surface integrin binding. May serve as a communication link between the dermal fibroblast cell surface and its extracellular matrix environment. Enhances TGFB1 activity. Inhibits cell proliferation. Accelerates collagen fibril formation, and stabilizes collagen fibrils against low-temperature dissociation ().