Western Blot (WB)
(Western blot analysis of DPT antibody (Center) in HL60 cell line lysates (35ug/lane). DPT (arrow) was detected using the purified Pab.)
Rabbit anti-Human DPT Polyclonal Antibody | anti-DPT antibody
DPT, ID (Dermatopontin, Tyrosine-rich Acidic Matrix Protein, TRAMP)
Purified by ammonium sulfate precipitation.
Purified by ammonium sulfate precipitation.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Western Blot (WB)
(Western blot analysis of DPT antibody (Center) in HL60 cell line lysates (35ug/lane). DPT (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of DPT antibody (Center) in HL60 cell line lysates (35ug/lane). DPT (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain with DPT Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain with DPT Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of NCI-H292 cells using DPT Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of NCI-H292 cells using DPT Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Immunofluorescence (IF)
(Immunofluorescence analysis of DPT Antibody (Center) with paraffin-embedded human brain tissue . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.)
Immunofluorescence (IF)
(Immunofluorescence analysis of DPT Antibody (Center) with paraffin-embedded human brain tissue . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.)
NCBI and Uniprot Product Information
NCBI Description
Dermatopontin is an extracellular matrix protein with possible functions in cell-matrix interactions and matrix assembly. The protein is found in various tissues and many of its tyrosine residues are sulphated. Dermatopontin is postulated to modify the behavior of TGF-beta through interaction with decorin. [provided by RefSeq, Jul 2008]
Uniprot Description
DPT: Seems to mediate adhesion by cell surface integrin binding. May serve as a communication link between the dermal fibroblast cell surface and its extracellular matrix environment. Enhances TGFB1 activity. Inhibits cell proliferation. Accelerates collagen fibril formation, and stabilizes collagen fibrils against low-temperature dissociation. Belongs to the dermatopontin family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 1q12-q23
Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space
Biological Process: negative regulation of cell proliferation; collagen fibril organization; cell adhesion
Research Articles on DPT
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Product Notes
The DPT dpt (Catalog #AAA627786) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DPT, ID (Dermatopontin, Tyrosine-rich Acidic Matrix Protein, TRAMP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DPT can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:1,000 Western Blot: 1:50-1:100. Researchers should empirically determine the suitability of the DPT dpt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DPT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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