Rabbit anti-Human Dopamine Receptor, D2S, L Polyclonal Antibody | anti-DRD2 antibody

Dopamine Receptor, D2S, L

Cat. Num. AAA611864

• Gene Names: DRD2; D2R; D2DR
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Serum; Serum.
• Synonyms: Dopamine Receptor; D2S; L; Polyclonal Antibody; Anti -Dopamine Receptor; anti-DRD2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human D2R. No significant sequence homology is seen with other dopamine receptors (D1 and D3-D5). Species sequence homology: Bovine, canine-100%; xenopus-50%.
• Purity/Purification: Serum; Serum.
• Form/Format: Supplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol.

• Applicable Applications for anti-DRD2 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1:1000-1:5000 (ECL) The predicted size of D2 form is approx. 50kD. However, additional bands at approx. 100kD, 68kD, and 50kD, and others are typically seen in various tissues (2-3). Approx. 100-400ug membrane protein/lane should be loaded to visualize various bands.; ELISA: 1:10,000-50,000. Use 1ug/ml to coat ELISA plates.
• Immunogen: A 28 AA synthetic peptide sequence from the human D2 R, within the cytoplasmic loop # 3 (KLH). The 28 AA sequence is shared by both the long and short form.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-DRD2 antibody

Dopamine D2 receptor is known to exist in the short (D2S) and the long (D2L)forms that are encoded by splice variants of a single gene and differ only by the presence of an additional 29aa in the intracellular, cytoplasmic loop 3 (1). D2L receptor is a 446aa, G-protein coupled, and transmembrane receptor protein.

Product Categories/Family for anti-DRD2 antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 17986270
• NCBI GeneID: 1813
• NCBI Accession #: NP_057658.2
• NCBI GenBank Nucleotide #: NM_016574.3
• UniProt Accession #: P14416; Q9NZR3; Q9UPA9
• Molecular Weight: 50,619 Da
• NCBI Official Full Name: D(2) dopamine receptor isoform short
• NCBI Official Synonym Full Names: dopamine receptor D2
• NCBI Official Symbol: DRD2
• NCBI Official Synonym Symbols: D2R; D2DR
• NCBI Protein Information: D(2) dopamine receptor; D(2) dopamine receptor; dopamine D2 receptor; dopamine receptor D2 isoform; seven transmembrane helix receptor
• UniProt Protein Name: D(2) dopamine receptor
• Protein Family: D(2) dopamine receptor
• UniProt Gene Name: DRD2
• UniProt Entry Name: DRD2_HUMAN

NCBI Description

This gene encodes the D2 subtype of the dopamine receptor. This G-protein coupled receptor inhibits adenylyl cyclase activity. A missense mutation in this gene causes myoclonus dystonia; other mutations have been associated with schizophrenia. Alternative splicing of this gene results in two transcript variants encoding different isoforms. A third variant has been described, but it has not been determined whether this form is normal or due to aberrant splicing. [provided by RefSeq, Jul 2008]

Uniprot Description

DRD2: Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Defects in DRD2 are associated with dystonia type 11 (DYT11); also known as alcohol-responsive dystonia. DYT11 is a myoclonic dystonia. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT11 is characterized by involuntary lightning jerks and dystonic movements and postures alleviated by alcohol. Inheritance is autosomal dominant. The age of onset, pattern of body involvement, presence of myoclonus and response to alcohol are all variable. Belongs to the G-protein coupled receptor 1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: synaptic vesicle membrane; endocytic vesicle; axon; integral to plasma membrane; dendrite; postsynaptic density; acrosome; plasma membrane; dendritic spine; perikaryon; nerve terminal; lateral plasma membrane

Molecular Function: dopamine D2 receptor-like receptor activity; ionotropic glutamate receptor binding; identical protein binding; protein binding; protein homodimerization activity; potassium channel regulator activity; protein heterodimerization activity; dopamine binding; drug binding

Biological Process: response to nicotine; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); prepulse inhibition; positive regulation of dopamine uptake; response to toxin; positive regulation of receptor internalization; thermoregulation; positive regulation of multicellular organism growth; regulation of phosphoprotein phosphatase activity; dopamine metabolic process; adult walking behavior; negative regulation of insulin secretion; dopamine receptor, adenylate cyclase inhibiting pathway; protein localization; phosphatidylinositol metabolic process; negative regulation of blood pressure; response to drug; response to inactivity; response to light stimulus; positive regulation of neuroblast proliferation; cerebral cortex GABAergic interneuron migration; negative regulation of synaptic transmission, glutamatergic; regulation of sodium ion transport; arachidonic acid secretion; regulation of synaptic transmission, GABAergic; positive regulation of growth hormone secretion; G-protein coupled receptor internalization; reduction of cytosolic calcium ion concentration; activation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; regulation of long-term neuronal synaptic plasticity; synaptic transmission, dopaminergic; peristalsis; branching morphogenesis of a nerve; negative regulation of circadian sleep/wake cycle, sleep; response to morphine; locomotory behavior; behavioral response to ethanol; orbitofrontal cortex development; negative regulation of cell proliferation; synaptogenesis; behavioral response to cocaine; visual learning; adenohypophysis development; feeding behavior; nerve-nerve synaptic transmission; circadian regulation of gene expression; response to axon injury; negative regulation of dopamine secretion; response to iron ion; negative regulation of cell migration; associative learning; positive regulation of cytokinesis; grooming behavior; negative regulation of protein secretion; Wnt receptor signaling pathway; negative regulation of protein kinase B signaling cascade; striatum development; regulation of potassium ion transport; regulation of cAMP metabolic process; regulation of heart rate; sensory perception of smell; negative regulation of adenylate cyclase activity; response to amphetamine; regulation of dopamine uptake; auditory behavior; response to cocaine; cellular calcium ion homeostasis; regulation of systemic arterial blood pressure by neurological process; negative regulation of dopamine receptor signaling pathway; long-term memory; pigmentation; axonogenesis; positive regulation of G-protein coupled receptor protein signaling pathway; release of sequestered calcium ion into cytosol; response to hypoxia; regulation of synapse structural plasticity; dopamine receptor, phospholipase C activating pathway

Disease: Myoclonic Dystonia