Testing Data
Rabbit CD3 zeta Polyclonal Antibody | anti-CD3 zeta antibody
Rabbit anti CD3 zeta Polyclonal antibody
Reactivity
Human, Rat, Mouse
Applications
immunoassay, Radioimmunoassay, Western Blot, Immunoprecipitation, Immunohistochemistry, Flow Cytometry
Purity
The Rabbit IgG is purified by Epitope-specific Affinity Purification.
Synonyms
CD3 zeta; Polyclonal Antibody; Rabbit anti CD3 zeta Polyclonal antibody; Rabbit anti-CD3; anti-CD3 zeta antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Specificity
This antibody recognizes human CD3 zeta protein. It cross-reacts to human, mouse and rat. The other species was not tested.
Purity/Purification
The Rabbit IgG is purified by Epitope-specific Affinity Purification.
Form/Format
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
Concentration
Size: 100 ug/200 ul (varies by lot)
Applicable Applications for anti-CD3 zeta antibody
Radioimmunoassay, Western Blot, Immunoprecipitation, Immunohistochemistry, Flow Cytometry
Application Notes
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 2-5 ug/ml
Flow Cytometry: 5-10 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 2-5 ug/ml
Flow Cytometry: 5-10 ug/ml
Antigen Preparation
A synthetic peptide corresponding to the N-terminus of human CD3 zeta. This sequence is identical among human, rat and mouse species.
Positive Control
Jurkat Cell, Tonsil Tissue
Cellular Location
Membrane/Cytoplasmic
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Testing Data
Testing Data
Related Product Information for anti-CD3 zeta antibody
CD3 is a glycoprotein consisting of up to five polypeptide chains (gp20, gp26, p16, p20, p28). It forms three dimmers: a heterodimer of gamma and epsilon chain, a heterodimer of delta and epsilon chains and a homodimer of two zeta chains or a heterodimer of zeta and eta chains. CD3 is required for proper assembly, trafficking and surface expression of TCR complex. CD3 is expressed by Thymocytes in a developmentally regulated manner and by all mature T cells. CD3 is involved in T cell activation, proliferation and apoptosis pathway. This protein is present on virtually all peripheral blood T cells and Thymocytes. The CD3 zeta plays an important role in coupling antigen recognition to several intracellular signal transduction pathway. Low expression of the antigen results in impaired immune response.
References
Kearse KP. Calnexin associates with monomeric and oligomeric (disulfidelinked) CD3delta proteins in murine T lymphocytes. J Biol Chem. (1998) 273 (23):141527.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~20 kDa
NCBI Official Full Name
CD3 zeta
NCBI Official Symbol
LOC100305023
NCBI Protein Information
CD3 zeta
Similar Products
Product Notes
The CD3 zeta (Catalog #AAA462233) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti CD3 zeta Polyclonal antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD3 zeta can be used in a range of immunoassay formats including, but not limited to, Radioimmunoassay, Western Blot, Immunoprecipitation, Immunohistochemistry, Flow Cytometry. Western Blot: 0.1-1 ug/ml ELISA: 0.01-0.1 ug/ml Immunoprecipitation: 2-5 ug/ml Immunohistochemistry: 2-5 ug/ml Flow Cytometry: 5-10 ug/ml. Researchers should empirically determine the suitability of the CD3 zeta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD3 zeta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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