Donkey Anti-Rabbit IgG (H+L) Secondary Antibody
Donkey Anti-Rabbit IgG (H+L), Human/Rat/Mouse ads-BIOT
Reactivity
Rabbit
Applications
ELISA, Immunoblot, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity chromatography on Rabbit IgG covalently linked to agarose.
Synonyms
Donkey Anti-Rabbit IgG (H+L); Polyclonal Secondary Antibody; Human/Rat/Mouse ads-BIOT; Donkey Anti-Rabbit IgG(H+L); Human/Rat/Mouse ads - Biotin; Donkey Anti-Rabbit IgG (H+L) secondary antibody
Host
Donkey
Reactivity
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
Reacts with the heavy and light chains of Rabbit IgG as demonstrated by ELISA. May also react with the light chains of other Rabbit immunoglobulins.
Purity/Purification
Affinity chromatography on Rabbit IgG covalently linked to agarose.
Form/Format
Biotin (BIOT) Conjugate
Applicable Applications for Donkey Anti-Rabbit IgG (H+L) secondary antibody
ELISA; Immunoblot; Immunofluorescence; Immunohistochemistry; Western Blot
Application Notes
Immunofluorescence Suggested Dilution: <= 1 ug/10^6 cells
ELISA Suggested Dilution: 1:5,000-1:20,000
ELISA Suggested Dilution: 1:5,000-1:20,000
Source
Pooled antisera from donkey hyperimmunized with rabbit IgG.
Cross Absorption
Minimal cross reaction to Human, Rat and Mouse serum proteins
Characterization
To ensure lot-to-lot consistency, each batch of product is tested by ELISA and/or Fluorescent ELISA for conformance to characteristics of a standard reference reagent.
Preparation and Storage
The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C.
The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C.
The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C.
The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C.
The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
Product Categories/Family for Donkey Anti-Rabbit IgG (H+L) secondary antibody
Similar Products
Product Notes
The Donkey Anti-Rabbit IgG (H+L) (Catalog #AAA674966) is a Secondary Antibody produced from Donkey and is intended for research purposes only. The product is available for immediate purchase. The Donkey Anti-Rabbit IgG (H+L), Human/Rat/Mouse ads-BIOT reacts with Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's Donkey Anti-Rabbit IgG (H+L) can be used in a range of immunoassay formats including, but not limited to, ELISA; Immunoblot; Immunofluorescence; Immunohistochemistry; Western Blot. Immunofluorescence Suggested Dilution: <= 1 ug/10^6 cells ELISA Suggested Dilution: 1:5,000-1:20,000. Researchers should empirically determine the suitability of the Donkey Anti-Rabbit IgG (H+L) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Donkey Anti-Rabbit IgG (H+L), Polyclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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