Western Blot (WB)
(Staining (0.5 ug/ml) of K562 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Goat RNF2/dinG Polyclonal Antibody | anti-RNF2 antibody
Goat anti-RNF2/dinG Antibody
Western Blot: Approx.37-38kDa band observed in lysates of cell lines of NIH3T3 and K562 (calculated MW of 37.7kDa according to NP_009143.1). Recommended concentration: 0.3-1ug/ml.
Western Blot (WB)
(Staining (0.5 ug/ml) of K562 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(Staining (0.5 ug/ml) of K562 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. The protein encoded by this gene is one of the PcG proteins. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin-conjugating enzyme, and possess ubiquitin ligase activity. [provided by RefSeq, Jul 2008]
Uniprot Description
RNF2: E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation. Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. E3 ubiquitin- protein ligase activity is enhanced by BMI1/PCGF4. Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity. Component of chromatin-associated Polycomb (PcG) complexes. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2/RING2, MBLR, L3MBTL2 and YAF2. Component of a PRC1-like complex. Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components C17orf49, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with RYBP, HIP2 and TFCP2. Association to the chromosomal DNA is cell-cycle dependent. Component of repressive BCOR complex containing Polycomb group subcomplex at least composed of RYBP, PCGF1, BCOR and RING1. Interacts with Interacts with PCGF2, CBX4, CBX6, CBX7 and CBX8. Interacts with CBX2, BMI and PHC2. Interacts with RYBP, HIP2 and TFCP2.
Protein type: Ubiquitin conjugating system; EC 6.3.2.-; Ubiquitin ligase; Ligase; EC 6.3.2.19
Chromosomal Location of Human Ortholog: 1q25.3
Cellular Component: euchromatin; nuclear body; nucleoplasm; nucleus; PcG protein complex; sex chromatin; ubiquitin ligase complex
Molecular Function: chromatin binding; ligase activity; protein binding; ubiquitin-protein ligase activity; zinc ion binding
Biological Process: anterior/posterior axis specification; gastrulation with mouth forming second; germ cell development; mitotic cell cycle; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; protein sumoylation; response to retinoic acid; spinal cord development; transcription, DNA-dependent
Research Articles on RNF2
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Product Notes
The RNF2 rnf2 (Catalog #AAA420926) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-RNF2/dinG Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's RNF2/dinG can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 4000. Western Blot: Approx.37-38kDa band observed in lysates of cell lines of NIH3T3 and K562 (calculated MW of 37.7kDa according to NP_009143.1). Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the RNF2 rnf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: PMELYYAPTK EHK. It is sometimes possible for the material contained within the vial of "RNF2/dinG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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