Rabbit anti-Human DOK4 Polyclonal Antibody | anti-DOK4 antibody

DOK4, CT (Docking Protein 4, Downstream Of Tyrosine Kinase 4, Insulin Receptor Substrate 5, IRS-5, IRS5)

Cat. Num. AAA629960

• Gene Names: DOK4; FLJ10488
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: DOK4; Polyclonal Antibody; CT (Docking Protein 4; Downstream Of Tyrosine Kinase 4; Insulin Receptor Substrate 5; IRS-5; IRS5); Anti -DOK4; anti-DOK4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DOK4.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-DOK4 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:50-1:200; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the C-terminal region of human DOK4 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of DOK4 (C-term) (arrow) using DOK4 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DOK4 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-DOK4 antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).

Product Categories/Family for anti-DOK4 antibody

Antibodies; Abs to Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 20070302
• NCBI GeneID: 55715
• NCBI Accession #: NP_060580.2
• NCBI GenBank Nucleotide #: NM_018110.3
• UniProt Accession #: Q8TEW6; O75209; Q9BTP2; Q9NVV3
• Molecular Weight: 37,028 Da
• NCBI Official Full Name: docking protein 4
• NCBI Official Synonym Full Names: docking protein 4
• NCBI Official Symbol: DOK4
• NCBI Official Synonym Symbols: FLJ10488
• NCBI Protein Information: docking protein 4; IRS5; IRS-5; OTTHUMP00000164664; insulin receptor substrate 5; downstream of tyrosine kinase 4
• UniProt Protein Name: Docking protein 4
• Protein Family: Docking protein
• UniProt Gene Name: DOK4
• UniProt Entry Name: DOK4_HUMAN

Uniprot Description

DOK4: docking proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK4 functions in Ret-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway. Putative link with downstream effectors of Ret in neuronal differentiation. May be involved in the regulation of the immune response induced by T-cells. Interacts with Ret and TIE2. Interaction with Ret is mediated through the PTB domain and requires phosphorylation of Ret Y1062.

Protein type: Cell development/differentiation; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 16q21

Molecular Function: protein binding; receptor signaling protein activity; insulin receptor binding

Biological Process: nervous system development; MAPKKK cascade; transmembrane receptor protein tyrosine kinase signaling pathway