Rabbit BRE Polyclonal Antibody | anti-BRE antibody

BRE Polyclonal Antibody

Cat. Num. AAA3007635

• Gene Names: BABAM2; BRE; BRCC4; BRCC45
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: BRE; Polyclonal Antibody; BRE Polyclonal Antibody; BRISC and BRCA1-A complex member 2; BRCA1-A complex subunit BRE; BRCA1/BRCA2-containing complex subunit 45; Brain and reproductive organ-expressed protein; BABAM2; BRCC45; anti-BRE antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: BRE polyclonal antibody detects endogenous levels of BRE protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 379

• Applicable Applications for anti-BRE antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 330-380 of Human BRE.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of BRE polyclonal antibody at 1:500 dilutionLane1:L02 whole cell lysate (40ug) Lane2:HCT116 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:PC12 whole cell lysate (20ug) Lane5:CT26 whole cell lysate (40ug))

Related Product Information for anti-BRE antibody

The breast cancer susceptibility gene, BRCA1, codes for an E3 ubiquitin ligase that functions in the maintenance of genome stability through regulation of the DNA damage response and DNA repair. BRCA1 protein forms at least three distinct complexes (BRCA1 A, B, and C) with other DNA repair proteins, and these interactions are vital for regulation of BRCA1 function. The BRCA1 A complex includes Rap80, BRCC36, Abraxas, MERIT40/NBA1, and BRE/BRCC45 and functions in G2/M phase checkpoint control. MERIT40 and BRE maintain the stability of both the BRCA1 A complex and the cytoplasmic BRISC complex, which contains BRCC36 and ABRO1 but not BRCA1. Researchers have shown that the expression level of BRE is related to patient survival in breast cancer, and it may predict a favorable outcome in acute myeloid leukemia (AML). Studies have also shown that BRE is overexpressed in human hepatocellular carcinoma and that overexpression of BRE can cause resistance to apoptotic signaling and promote tumor growth.

NCBI and Uniprot Product Information

• NCBI GI #: 387912573
• NCBI GeneID: 9577
• NCBI Accession #: NP_001248769.1
• NCBI GenBank Nucleotide #: NP_001248769.1
• UniProt Accession #: Q9NXR7
• Molecular Weight: ~ 45kDa
• NCBI Official Full Name: BRISC and BRCA1-A complex member 2 isoform 4
• NCBI Official Synonym Full Names: BRISC and BRCA1 A complex member 2
• NCBI Official Symbol: BABAM2
• NCBI Official Synonym Symbols: BRE; BRCC4; BRCC45
• NCBI Protein Information: BRISC and BRCA1-A complex member 2
• UniProt Protein Name: BRCA1-A complex subunit BRE
• Protein Family: Brevinin
• UniProt Gene Name: BRE
• UniProt Synonym Gene Names: BRCC45
• UniProt Entry Name: BRE_HUMAN

NCBI Description

This gene encodes an anti-apoptotic, death receptor-associated protein that interacts with tumor necrosis factor-receptor-1. The encoded protein acts as an adapter in several protein complexes, including the BRCA1-A complex and the BRISC complex. The BRCA1-A complex possesses ubiquitinase activity and targets sites of double strand DNA breaks, while the BRISC complex exhibits deubiquitinase activity and is involved in mitotic spindle assembly. This gene is upregulated in several types of cancer. [provided by RefSeq, Jun 2016]

Uniprot Description

BRE: Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it acts as an adapter that bridges the interaction between BABAM1/NBA1 and the rest of the complex, thereby being required for the complex integrity and modulating the E3 ubiquitin ligase activity of the BRCA1-BARD1 heterodimer. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. May play a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins. May also act as a death receptor-associated anti- apoptotic protein, which inhibits the mitochondrial apoptotic pathway. May regulate TNF-alpha signaling through its interactions with TNFRSF1A; however these effects may be indirect. Belongs to the BRE family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage; Ubiquitin conjugating system; Cytokine; Apoptosis

Chromosomal Location of Human Ortholog: 2p23.2

Cellular Component: cytoplasm; nuclear ubiquitin ligase complex; nucleus

Molecular Function: protein binding; peroxisome targeting sequence binding; polyubiquitin binding; tumor necrosis factor receptor binding

Biological Process: positive regulation of DNA repair; apoptosis; double-strand break repair; response to ionizing radiation; chromatin modification; G2/M transition DNA damage checkpoint; signal transduction; response to DNA damage stimulus