Western Blot
(Western Blot analysis using Goat anti Human Kappa Chain50ng of Human Kappa Chain (Lane 2) and 50ng of Human Lambda Chain (Lane 3) was run on a 4-20% gel and transferred to 0. 45 um nitrocellulose. After blocking with 1% BSA-TTBS blocking buffer for 30 min at 20 deg C, Goat anti-Human Kappa chain (FITC) F(ab')2 secondary antibody was used at 1:1000 in blocking buffer. )
Kappa Chain Secondary Antibody
Goat anti Human Kappa Chain (Fab'2) (FITC)
Reactivity
Human kappa chain
Applications
ELISA, Flow Cytometry, Functional Assay, Immunofluorescence
Purity
Goat anti Human Kappa Chain (Fab'2) (FITC) was purified by affinity chromatography.
Synonyms
Kappa Chain; Goat anti Human Kappa Chain (Fab'2) (FITC); Goat anti Human Kappa Chain secondary (Fab'2) (FITC); Kappa Chain secondary antibody
Host
Goat
Reactivity
Human kappa chain
Specificity
Human kappa chain
Purity/Purification
Goat anti Human Kappa Chain (Fab'2) (FITC) was purified by affinity chromatography.
Form/Format
Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. Immunoglobulin and protease free.
Applicable Applications for Kappa Chain secondary antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes
ELISA: 1:10,000-1:50,000
FC: 1:500-1:2,500
IF: 1:1,000-1:5,000
FC: 1:500-1:2,500
IF: 1:1,000-1:5,000
Biohazard Information
This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Tag/Conjugate
FITC
Immunogen
Goat anti-human kappa chain (Fab'2) (FITC) was raised in goat using human kappa light chain as the immunogen.
Preparation and Storage
Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot
(Western Blot analysis using Goat anti Human Kappa Chain50ng of Human Kappa Chain (Lane 2) and 50ng of Human Lambda Chain (Lane 3) was run on a 4-20% gel and transferred to 0. 45 um nitrocellulose. After blocking with 1% BSA-TTBS blocking buffer for 30 min at 20 deg C, Goat anti-Human Kappa chain (FITC) F(ab')2 secondary antibody was used at 1:1000 in blocking buffer. )
Western Blot
(Western Blot analysis using Goat anti Human Kappa Chain50ng of Human Kappa Chain (Lane 2) and 50ng of Human Lambda Chain (Lane 3) was run on a 4-20% gel and transferred to 0. 45 um nitrocellulose. After blocking with 1% BSA-TTBS blocking buffer for 30 min at 20 deg C, Goat anti-Human Kappa chain (FITC) F(ab')2 secondary antibody was used at 1:1000 in blocking buffer. )
Related Product Information for Kappa Chain secondary antibody
Goat anti Human Kappa Chain secondary antibody (Fab'2) (FITC)
Product Categories/Family for Kappa Chain secondary antibody
Similar Products
Product Notes
The Kappa Chain (Catalog #AAA538823) is a Secondary Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti Human Kappa Chain (Fab'2) (FITC) reacts with Human kappa chain and may cross-react with other species as described in the data sheet. AAA Biotech's Kappa Chain can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF). ELISA: 1:10,000-1:50,000 FC: 1:500-1:2,500 IF: 1:1,000-1:5,000. Researchers should empirically determine the suitability of the Kappa Chain for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Kappa Chain, Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
