Rabbit DOCK3/MOCA Polyclonal Antibody | anti-DOCK3/MOCA antibody

DOCK3/MOCA Antibody

Cat. Num. AAA9611707

• Gene Names: DOCK3; PBP; MOCA; NEDIDHA
• Reactivity: Human, Mouse; Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
• Applications: Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: DOCK3/MOCA; Polyclonal Antibody; DOCK3/MOCA Antibody; Dedicator of cytokinesis protein 3; DOCK 3; MOCA; Modifier of cell adhesion; Presenilin binding protein; anti-DOCK3/MOCA antibody

• Host: Rabbit
• Reactivity: Human, Mouse; Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: DOCK3; MOCA Antibody detects endogenous levels of DOCK3;MOCA.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 2030

• Applicable Applications for anti-DOCK3/MOCA antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human DOCK3;MOCA.
• Tissue Specificity: In normal brains, it is localized in the neuropil, and occasionally in the pyramidal cells, while in Alzheimer disease brains, it is associated with neurofibrillary tangles.
• Fragment: Fab Fragment
• Conjugate: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from SH-SY5Y cells, using DOCK3;MOCA Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-DOCK3/MOCA antibody

Description: Potential guanine nucleotide exchange factor (GEF). GEF proteins activate some small GTPases by exchanging bound GDP for free GTP. Its interaction with presenilin proteins as well as its ability to stimulate Tau/MAPT phosphorylation suggest that it may be involved in Alzheimer disease. Ectopic expression in nerve cells decreases the secretion of amyloid-beta APBA1 protein and lowers the rate of cell-substratum adhesion, suggesting that it may affect the function of some small GTPase involved in the regulation of actin cytoskeleton or cell adhesion receptors (By similarity).

Subunit Structure: Interacts with presenilin proteins PSEN1 and PSEN2. Interacts with CRK (By similarity).

Similarity: The DHR-2 domain may mediate some GEF activity. Belongs to the DOCK family.

NCBI and Uniprot Product Information

• NCBI GI #: 31415870
• NCBI GeneID: 1795
• NCBI Accession #: NP_004938.1
• NCBI GenBank Nucleotide #: NP_004938.1
• UniProt Accession #: Q8IZD9
• Molecular Weight: Observed: 233 kDa; Predicted: 234 kDa
• NCBI Official Full Name: dedicator of cytokinesis protein 3
• NCBI Official Synonym Full Names: dedicator of cytokinesis 3
• NCBI Official Symbol: DOCK3
• NCBI Official Synonym Symbols: PBP; MOCA; NEDIDHA
• NCBI Protein Information: dedicator of cytokinesis protein 3
• UniProt Protein Name: Dedicator of cytokinesis protein 3
• UniProt Gene Name: DOCK3
• UniProt Synonym Gene Names: KIAA0299; MOCA; PBP

NCBI Description

This gene is specifically expressed in the central nervous system (CNS). It encodes a member of the DOCK (dedicator of cytokinesis) family of guanine nucleotide exchange factors (GEFs). This protein, dedicator of cytokinesis 3 (DOCK3), is also known as modifier of cell adhesion (MOCA) and presenilin-binding protein (PBP). The DOCK3 and DOCK1, -2 and -4 share several conserved amino acids in their DHR-2 (DOCK homology region 2) domains that are required for GEF activity, and bind directly to WAVE proteins [Wiskott-Aldrich syndrome protein (WASP) family Verprolin-homologous proteins] via their DHR-1 domains. The DOCK3 induces axonal outgrowth in CNS by stimulating membrane recruitment of the WAVE complex and activating the small G protein Rac1. This gene is associated with an attention deficit hyperactivity disorder-like phenotype by a complex chromosomal rearrangement. [provided by RefSeq, Aug 2010]

Uniprot Description

Potential guanine nucleotide exchange factor (GEF). GEF proteins activate some small GTPases by exchanging bound GDP for free GTP. Its interaction with presenilin proteins as well as its ability to stimulate Tau/MAPT phosphorylation suggest that it may be involved in Alzheimer disease. Ectopic expression in nerve cells decreases the secretion of beta-amyloid APBA1 protein and lowers the rate of cell-substratum adhesion, suggesting that it may affect the function of some small GTPase involved in the regulation of actin cytoskeleton or cell adhesion receptors ().