Goat anti-Goat, Human DOCK2 Polyclonal Antibody | anti-DOCK2 antibody

DOCK2 (Dedicator of Cytokinesis Protein 2, KIAA0209)

Cat. Num. AAA626794

• Gene Names: DOCK2; FLJ46592; KIAA0209
• Reactivity: Goat, Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: DOCK2; Polyclonal Antibody; DOCK2 (Dedicator of Cytokinesis Protein 2; KIAA0209); Anti -DOCK2 (Dedicator of Cytokinesis Protein 2; anti-DOCK2 antibody

• Host: Goat
• Reactivity: Goat, Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DOCK2. Species sequence homology: mouse (96%).
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute in PBS, 0.02% sodium azide.

• Applicable Applications for anti-DOCK2 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot:1g/ml
• Immunogen: Purified, E. coli-derived recombinant human DOCK2 (aa420-642; Accession #Q92608).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-DOCK2 antibody

DOCK2 (Dedicator of cytokinesis protein 2) is a member of the DOCK-A family, DOCK180 superfamily of proteins. Its predicted molecular weight is 210kD, although it may run approximately at 190kD in SDS-PAGE. It is found in hematopoietic cell types, and regulates the activity of the GTPases Rac-1 and -2. Human DOCK2 is 1830aa in length. It contains one SH3 domain (aa8-69), a DHR-1 region that localizes DOCK2 to the plasma membrane (aa420-662), and a DHR-2 domain that binds to nucleotide-free GTPases and shows GEF activity (aa1115-1621). There are two isoform variants, one that shows a 38aa substitution for Tyr1405, and a second that shows a start site at Met450 with accompanying deletions of aa462-494 and 630-766, and a 22aa substitution for aa934-1830. Over aa420-642, human DOCK2 shares 96% aa identity to mouse DOCK2.

Product Categories/Family for anti-DOCK2 antibody

Antibodies; Abs to Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 31377468
• NCBI GeneID: 1794
• NCBI Accession #: NP_004937.1
• NCBI GenBank Nucleotide #: NM_004946.2
• UniProt Accession #: Q92608; Q2M3I0; Q96AK7; Q5XG91; Q6ZNS5
• Molecular Weight: 211,948 Da
• NCBI Official Full Name: dedicator of cytokinesis protein 2
• NCBI Official Synonym Full Names: dedicator of cytokinesis 2
• NCBI Official Symbol: DOCK2
• NCBI Official Synonym Symbols: FLJ46592; KIAA0209
• NCBI Protein Information: dedicator of cytokinesis protein 2; OTTHUMP00000160994; OTTHUMP00000223166; dedicator of cyto-kinesis 2
• UniProt Protein Name: Dedicator of cytokinesis protein 2
• Protein Family: Dedicator of cytokinesis protein
• UniProt Gene Name: DOCK2
• UniProt Synonym Gene Names: KIAA0209
• UniProt Entry Name: DOCK2_HUMAN

NCBI Description

The DOCK2 gene encodes a hematopoietic cell-specific CDM family protein that is indispensable for lymphocyte chemotaxis.[supplied by OMIM]

Uniprot Description

DOCK2: a hematopoietic cell-specific CDM family protein that is indispensable for lymphocyte chemotaxis. Plays a role in T cell receptor-induced activation of Rac2 and IL-2 transcription. Interacts with RAC, CRKL, and CD3-zeta. Association with ELMO1 is critical for DOCK2-mediated Rac activation. Two alternatively-spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; GAPs; GAPs, Rac/Rho; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 5q35.1

Cellular Component: cytoskeleton; membrane; endomembrane system; cytosol

Molecular Function: protein binding; Rac guanyl-nucleotide exchange factor activity; T cell receptor binding

Biological Process: alpha-beta T cell proliferation; viral reproduction; formation of immunological synapse; myeloid dendritic cell activation during immune response; small GTPase mediated signal transduction; positive regulation of phagocytosis; establishment of T cell polarity; lipid raft polarization; chemotaxis; actin cytoskeleton organization and biogenesis; negative thymic T cell selection; regulation of defense response to virus by virus; positive thymic T cell selection

Disease: Immunodeficiency 40