Western Blot (WB)
(Anti-DNTT rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:58 kDa)
Rabbit anti-Human Dntt Polyclonal Antibody | anti-Dntt antibody
Anti-Dntt Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
Dntt; Polyclonal Antibody; Anti-Dntt Antibody; Rabbit Polyclonal; Dntt Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-TDT Antibody; anti-Dntt antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human Dntt
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-Dntt antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
E Coli-derived Human Dntt fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human Dntt fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-DNTT rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:58 kDa)
Western Blot (WB)
(Anti-DNTT rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:58 kDa)
Related Product Information for anti-Dntt antibody
Terminal deoxynucleotidyl transferase (TdT) is a DNA polymerase expressed in immature, normal and neoplastic, lymphoid or haematopoietic cells and in neuroendocrine carcinomas, such as Merkel cell carcinoma and small-cell carcinoma. TdT inserts N-nucleotides to the V (D) J gene segment during rearrangements of genes, therefore, it plays a vital role in the development and variation of the immune system in vertebrates. V(D)J recombination creates diversity of antigen receptors in immune system. TdT is responsible for inserting nucleotides to the junctions of gene segment during V (D) J recombination. TdT gene is present in pre-B and pre-T cells and increases in some human leukemia's. For this reason, TdT is an effective biochemical marker for classifying leukemia's.
References
Tzorakoleftheraki SE, et al. (2017) Tdt expression in normal and neoplastic sebaceous cells. Histopathology 71 (6): 985-988.Gholami S, et al. (2017) Terminal deoxynucleotidyl transferase (tdt) inhibiti on of cord blood derived b and t cells expansion. Adv Pharm Bull 7 (2): 215-220.Brobeil A, et al. (2018) Expression of terminal deoxynucleotidyl transferase (tdt) in classical seminoma: A potential diagnostic pitfall. Virchows Arch 472 (3): 433-440.
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Product Notes
The Dntt (Catalog #AAA8110489) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Dntt Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Dntt can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the Dntt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Dntt, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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