Rabbit anti-Human Dnmt1-pS714 Polyclonal Antibody | anti-Dnmt1-pS714 antibody

Phospho-Dnmt1-pS714 Polyclonal Antibody

Cat. Num. AAA8527417

• Gene Names: DNMT1; AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN; m.HsaI
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity purification
• Synonyms: Dnmt1-pS714; Polyclonal Antibody; Phospho-Dnmt1-pS714 Polyclonal Antibody; AIM; DNMT; MCMT; CXXC9; HSN1E; FLJ16293; MGC104992; DNMT1; anti-Dnmt1-pS714 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 1632

• Applicable Applications for anti-Dnmt1-pS714 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A phospho specific peptide corresponding to residues surrounding S714 of human Dnmt1
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Related Product Information for anti-Dnmt1-pS714 antibody

Methylation of DNA at cytosine residues in mammalian cells is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting and development (1, 2). Three families of mammalian DNA methyltransferases have been identified: DNMT1, DNMT2 and DNMT3 (1, 2). DNMT1 is constitutively expressed in proliferating cells and functions as a maintenance methyltransferase, transferring proper methylation patterns to newly synthesized DNA during replication. DNMT3A and DNMT3B are strongly expressed in embryonic stem cells with reduced expression in adult somatic tissues. DNMT3A and DNMT3B function as de novo methyltransferases that methylate previously unmethylated regions of DNA. DNMT2 is expressed at low levels in adult somatic tissues and its inactivation affects neither de novo nor maintenance DNA methylation. DNMT1, DNMT3A and DNMT3B together form a protein complex that interacts with histone deacetylases (HDAC1, HDAC2, Sin3A), transcriptional repressor proteins (RB, TAZ-1) and heterochromatin proteins (HP1, SUV39H1), to maintain proper levels of DNA methylation and facilitate gene silencing (3-8). Improper DNA methylation contributes to diseased states such as cancer (1, 2). Hypermethylation of promoter CpG islands within tumor suppressor genes correlates with gene silencing and the development of cancer. In addition, hypomethylation of bulk genomic DNA correlates with and may contribute to the onset of cancer. DNMT1, DNMT3A and DNMT3B are over-expressed in many cancers, including acute and chronic myelogenous leukemias, in addition to colon, breast and stomach carcinomas (9-12).

References

1. Hermann, A. et al. (2004) Cell. Mol. Life Sci. 61, 2571-2587. 2. Turek-Plewa, J. and Jagodzi?ski, P.P. (2005) Cell. Mol. Biol. Lett. 10, 631-647. 3. Kim, G.D. et al. (2002) EMBO J. 21, 4183-4195. 4. Fuks, F. et al. (2001) EMBO J. 20, 2536-2544. 5. Geiman, T.M. et al. (2004) Biochem. Biophys. Res. Commun. 318, 544-555. 6. Rountree, M.R. et al. (2000) Nat. Genet. 25, 269-277. 7. Pradhan, S. and Kim, G.D. (2002) EMBO J. 21, 779-788. 8. Fuks, F. et al. (2003) Nucleic Acids Res. 31, 2305-2312. 9. Mizuno, S. et al. (2001) Blood 97, 1172-1179. 10. Robertson, K.D. et al. (1999) Nucleic Acids Res. 27, 2291-2298. 11. Xie, S. et al. (1999) Gene 236, 87-95. 12. Kanai, Y. et al. (2001) Int. J. Cancer 91, 205-212.

NCBI and Uniprot Product Information

• NCBI GI #: 195927037
• NCBI GeneID: 1786
• NCBI Accession #: NP_001124295.1
• NCBI GenBank Nucleotide #: NM_001130823.2
• UniProt Accession #: P26358; Q9UHG5; Q9ULA2; Q9UMZ6; A0AV63; B7ZLW6
• Molecular Weight: 144,465 Da
• NCBI Official Full Name: DNA (cytosine-5)-methyltransferase 1 isoform a
• NCBI Official Synonym Full Names: DNA methyltransferase 1
• NCBI Official Symbol: DNMT1
• NCBI Official Synonym Symbols: AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN; m.HsaI
• NCBI Protein Information: DNA (cytosine-5)-methyltransferase 1
• UniProt Protein Name: DNA (cytosine-5)-methyltransferase 1
• UniProt Gene Name: DNMT1
• UniProt Synonym Gene Names: AIM; CXXC9; DNMT; Dnmt1; DNA MTase HsaI; M.HsaI

NCBI Description

This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

Uniprot Description

Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306). Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306). Promotes tumor growth (PubMed:24623306).