Mouse ENO1 Monoclonal Antibody | anti-ENO1 antibody

ENO1 (Enolase 1, (alpha), ENO1L1, MBP-1, MPB1, NNE, PPH) (FITC)

Cat. Num. AAA6177532

• Gene Names: ENO1; NNE; PPH; MPB1; ENO1L1; HEL-S-17
• Applications: Western Blot
• Purity: Purified
• Synonyms: ENO1; Monoclonal Antibody; ENO1 (Enolase 1; (alpha); ENO1L1; MBP-1; MPB1; NNE; PPH) (FITC); Enolase 1; PPH; anti-ENO1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 90000
• Specificity: Recognizes ENO1.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
• Sequence Length: 434

• Applicable Applications for anti-ENO1 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: ENO1 (AAH22545, 1aa-434aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MSILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELRDNDKTRYMGKGVSKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKAKFAGRNFRNPLAK
• Conjugate: FITC
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.; ; FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ENO1 monoclonal antibody (M10), clone 9E4. Western Blot analysis of ENO1 expression in HepG2 (Cat # L019V1).)

Western Blot (WB)

(ENO1 monoclonal antibody (M10), clone 9E4. Western Blot analysis of ENO1 expression in MCF-7.)

Western Blot (WB)

(ENO1 monoclonal antibody (M10), clone 9E4. Western Blot analysis of ENO1 expression in human colon.)

Related Product Information for anti-ENO1 antibody

This gene encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome. [provided by RefSeq]

Product Categories/Family for anti-ENO1 antibody

Antibodies; Cancer Markers

NCBI and Uniprot Product Information

• NCBI GI #: 18490320
• NCBI GeneID: 2023
• NCBI Official Full Name: Enolase 1, (alpha)
• NCBI Official Synonym Full Names: enolase 1
• NCBI Official Symbol: ENO1
• NCBI Official Synonym Symbols: NNE; PPH; MPB1; ENO1L1; HEL-S-17
• NCBI Protein Information: alpha-enolase; c-myc promoter-binding protein-1
• Protein Family: Enolase

NCBI Description

This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]