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Western Blot (WB) (Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane: HT-29 cell, Primary antibody: 161629(DNASE2 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2MIN)

Rabbit anti-Human DNASE2 Polyclonal Antibody | anti-DNASE2 antibody

DNASE2 Rabbit pAb

Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity purified
Synonyms
DNASE2; Polyclonal Antibody; DNASE2 Rabbit pAb; DNL; DNL2; DNASE2A; anti-DNASE2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Affinity purified
Form/Format
-20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Applicable Applications for anti-DNASE2 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
WB: 1:200-1:1000
Immunogen
Synthetic peptide of human DNASE2
Conjugation
Unconjugated
Molecular Function
This gene encodes a member of the DNase family. The protein, located in the lysosome, hydrolyzes DNA under acidic conditions and mediates the breakdown of DNA during erythropoiesis and apoptosis. Two codominant alleles have been characterized, DNASE2*L (low activity) and DNASE2*H (high activity), that differ at one nucleotide in the promoter region. The DNASE2*H allele is represented in this record.
Modification
Unmodified
Cell Position
Lysosome
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane: HT-29 cell, Primary antibody: 161629(DNASE2 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2MIN)

Western Blot (WB) (Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane: HT-29 cell, Primary antibody: 161629(DNASE2 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2MIN)
Product Categories/Family for anti-DNASE2 antibody

NCBI and Uniprot Product Information

UniProt Accession #
Molecular Weight
40kDa
UniProt Protein Name
Deoxyribonuclease-2-alpha
Protein Family
UniProt Gene Name
DNASE2
UniProt Synonym Gene Names
DNASE2A; DNL2; DNase II alpha
UniProt Entry Name
DNS2A_HUMAN

Uniprot Description

DNASE2: Hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells. Belongs to the DNase II family.

Protein type: Deoxyribonuclease; EC 3.1.22.1

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: lysosome; intracellular

Molecular Function: DNA binding; deoxyribonuclease II activity

Biological Process: erythrocyte differentiation; DNA fragmentation during apoptosis; DNA metabolic process

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Product Notes

The DNASE2 dnase2 (Catalog #AAA4754798) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DNASE2 Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DNASE2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). WB: 1:200-1:1000. Researchers should empirically determine the suitability of the DNASE2 dnase2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNASE2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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