Rabbit DNAL1 Polyclonal Antibody | anti-DNAL1 antibody

DNAL1 Antibody

Cat. Num. AAA150344

• Gene Names: DNAL1; CILD16; C14orf168
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: DNAL1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: DNAL1; Polyclonal Antibody; DNAL1 Antibody; CILD16; C14orf168; Dynein light chain 1; axonemal; dynein; light chain 1; anti-DNAL1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: DNAL1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 190

• Applicable Applications for anti-DNAL1 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: DNAL1 antibody can be used for detection of DNAL1 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunocytochemistry starting at 2.5 mug/mL.
• Conjugate: Unconjugated
• Immunogen: DNAL1 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human DNAL1.
• Buffer: DNAL1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: DNAL1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of DNAL1 in 3T3 cell lysate with DNAL1 antibody at (A) 1 and (B) 2 μg/mL.)

Immunocytochemistry (IHC)

(Immunocytochemistry of DNAL1 in 3T3 cells with DNAL1 antibody at 2.5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of DNAL1 in 3T3 cells with DNAL1 antibody at 20 μg/mL.Green: DNAL1 Antibody (4733)Blue: DAPI staining)

Related Product Information for anti-DNAL1 antibody

DNAL1 Antibody: DNAL1 was identified as a potential candidate gene for primary ciliary dyskinesia (PCD), a genetically heterologous disorder characterized by chronic infections of the upper and lower airways that often leads to permanent lung damage, randomization of left/right body symmetry, and reduced fertility. DNAL1 is reported to be expressed solely in tissues carrying motile cilia for flagella and interacts with DNAH5, a protein that when mutated has been shown to result in PCD. It has been suggested that DNAL1 serves a regulatory function for DNAH5 activity in outer dynein arms of sperm flagella, respiratory cilia, and ependymal cilia. DNAL1 has also been recently identified as an HIV dependency factor (HDF), suggesting that DNAL1 may be an important drug target in HIV treatment. At least two isoforms of DNAL1 are known to exist.

NCBI and Uniprot Product Information

• NCBI GI #: 164607156
• NCBI GeneID: 83544
• NCBI Accession #: NP_113615
• NCBI GenBank Nucleotide #: NM_031427.3
• UniProt Accession #: Q4LDG9; Q5JPB7; Q9BS43; B2RD38
• Molecular Weight: 17,096 Da
• NCBI Official Full Name: dynein light chain 1, axonemal isoform 1
• NCBI Official Synonym Full Names: dynein, axonemal, light chain 1
• NCBI Official Symbol: DNAL1
• NCBI Official Synonym Symbols: CILD16; C14orf168
• NCBI Protein Information: dynein light chain 1, axonemal
• UniProt Protein Name: Dynein light chain 1, axonemal
• Protein Family: Dynein
• UniProt Gene Name: DNAL1
• UniProt Synonym Gene Names: C14orf168
• UniProt Entry Name: DNAL1_HUMAN

NCBI Description

This gene encodes an axonemal dynein light chain which functions as a component of the outer dynein arms complex. This complex acts as the molecular motor that provides the force to move cilia in an ATP-dependent manner. The encoded protein is expressed in tissues with motile cilia or flagella and may be involved in the movement of sperm flagella. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Jan 2011]

Uniprot Description

DNAL1: Defects in DNAL1 are the cause of primary ciliary dyskinesia type 16 (CILD16). A disorder characterized by abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia; reduced fertility is often observed in male patients due to abnormalities of sperm tails. Half of the patients exhibit randomization of left-right body asymmetry and situs inversus, due to dysfunction of monocilia at the embryonic node. Primary ciliary dyskinesia associated with situs inversus is referred to as Kartagener syndrome. Belongs to the dynein light chain LC1-type family. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 14q24.3

Disease: Ciliary Dyskinesia, Primary, 16