Western Blot (WB)
(Western blot analysis of DNAJB6 expression in U87-MG cell lysate ;, The lane on the left is treated with the antigen-specific peptide.)
Rabbit DNAJB6 Polyclonal Antibody | anti-DNAJB6 antibody
DNAJB6 Antibody
Gene Names
DNAJB6; DJ4; MRJ; DnaJ; HSJ2; HHDJ1; HSJ-2; MSJ-1; LGMD1D; LGMD1E; LGMDD1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
DNAJB6; Polyclonal Antibody; DNAJB6 Antibody; DJ4; DnaJ; DnaJ (Hsp40) homolog subfamily B member 6; DNAJ B6; DnaJ homolog subfamily B member 6; DnaJ like 2 protein; DNJB6_HUMAN; Heat shock protein J2; HHDJ1; HSJ-2; HSJ2; LGMD1E; MRJ; MSJ-1; MSJ1; anti-DNAJB6 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
DNAJB6 antibody detects endogenous levels of total DNAJB6
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
241
Applicable Applications for anti-DNAJB6 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide
Subcellular Location
Cytoplasm, Perinuclear Region. Nucleus. Cytoplasm, Myofibril, Sarcomere, Z Line.
Tissue Specificity
Widely expressed. Highest levels in testis and brain, and lower levels in heart, spleen, intestine, ovary, placenta, lung, kidney, pancreas, thymus, prostate, skeletal muscle, liver and leukocytes. In testis, expressed in germ cells in the earlier stages of differentiation pathway as well as in spermatids. In brain, expressed at a higher level in hippocampus and thalamus and a lower level in amygdala, substantia nigra, corpus callosum and caudate nucleus.
Predicted Cross Reactivity
Rabbit, Dog
Similarity
Rabbit (89%), Dog (89%)
Conjugation
Unconjugated
Western Blot (WB)
(Western blot analysis of DNAJB6 expression in U87-MG cell lysate ;, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of DNAJB6 expression in U87-MG cell lysate ;, The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-DNAJB6 antibody
Function: Plays an indispensable role in the organization of KRT8/KRT18 filaments. Acts as an endogenous molecular chaperone for neuronal proteins including huntingtin. Suppresses aggregation and toxicity of polyglutamine-containing, aggregation-prone proteins. Isoform B but not isoform A inhibits huntingtin aggregation. Has a stimulatory effect on the ATPase activity of HSP70 in a dose-dependent and time-dependent manner and hence acts as a co-chaperone of HSP70. Also reduces cellular toxicity and caspase-3 activity.
Subunit Structure: Homooligomer. Interacts with BAG3, HSPB8 and STUB1. Interacts with ALKBH1 (By similarity). Interacts with HSP70, KRT18 and PTTG. Isoform B interacts with histone deacetylases HDAC4, HDAC6, and SIRT2, HDAC activity is required for antiaggregation.
Similarity: The antiaggregation activity of isoform B resides in the serine-rich region and the C-terminus.
Subunit Structure: Homooligomer. Interacts with BAG3, HSPB8 and STUB1. Interacts with ALKBH1 (By similarity). Interacts with HSP70, KRT18 and PTTG. Isoform B interacts with histone deacetylases HDAC4, HDAC6, and SIRT2, HDAC activity is required for antiaggregation.
Similarity: The antiaggregation activity of isoform B resides in the serine-rich region and the C-terminus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 36 kDa
Predicted: 37 kDa
Predicted: 37 kDa
NCBI Official Full Name
dnaJ homolog subfamily B member 6 isoform b
NCBI Official Synonym Full Names
DnaJ heat shock protein family (Hsp40) member B6
NCBI Official Symbol
DNAJB6
NCBI Official Synonym Symbols
DJ4; MRJ; DnaJ; HSJ2; HHDJ1; HSJ-2; MSJ-1; LGMD1D; LGMD1E; LGMDD1
NCBI Protein Information
dnaJ homolog subfamily B member 6
UniProt Protein Name
DnaJ homolog subfamily B member 6
Protein Family
UniProt Gene Name
DNAJB6
UniProt Synonym Gene Names
HSJ2; MRJ; MSJ1; HSJ-2
NCBI Description
This gene encodes a member of the DNAJ protein family. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. This family member may also play a role in polyglutamine aggregation in specific neurons. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]
Uniprot Description
Plays an indispensable role in the organization of KRT8/KRT18 filaments. Acts as an endogenous molecular chaperone for neuronal proteins including huntingtin. Suppresses aggregation and toxicity of polyglutamine-containing, aggregation-prone proteins. Isoform B but not isoform A inhibits huntingtin aggregation. Has a stimulatory effect on the ATPase activity of HSP70 in a dose-dependent and time-dependent manner and hence acts as a co-chaperone of HSP70. Also reduces cellular toxicity and caspase-3 activity.
Research Articles on DNAJB6
Similar Products
Product Notes
The DNAJB6 dnajb6 (Catalog #AAA9604003) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DNAJB6 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DNAJB6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the DNAJB6 dnajb6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNAJB6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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