Rabbit anti-Human DNAJB5 Polyclonal Antibody | anti-DNAJB5 antibody

DNAJB5, CT (DNAJB5, HSC40, DnaJ homolog subfamily B member 5, Heat shock protein Hsp40-2, Heat shock protein Hsp40-3, Heat shock protein cognate 40) (AP)

Cat. Num. AAA6292685

• Gene Names: DNAJB5; Hsc40
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DNAJB5; Polyclonal Antibody; CT (DNAJB5; HSC40; DnaJ homolog subfamily B member 5; Heat shock protein Hsp40-2; Heat shock protein Hsp40-3; Heat shock protein cognate 40) (AP); Heat shock protein cognate 40; anti-DNAJB5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 382

• Applicable Applications for anti-DNAJB5 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: DNAJB5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 230-257 amino acids from the C-terminal region of human DNAJB5.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(DNAJB5 Antibody (C-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the DNAJB5 antibody detected the DNAJB5 protein (arrow).)

Related Product Information for anti-DNAJB5 antibody

DNAJB5 belongs to the evolutionarily conserved DNAJ/HSP40 protein family. For background information on the DNAJ family, see MIM 608375.

Product Categories/Family for anti-DNAJB5 antibody

Antibodies; Heat Shock Proteins

References

Ohtsuka, K., et al. Cell Stress Chaperones 5(2):98-112(2000). Chen, M.S., et al. Gene 238(2):333-341(1999). Adams, M.D., et al. Nature 377 (6547 SUPPL), 3-174 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 201862518
• NCBI GeneID: 25822
• NCBI Accession #: NP_001128476.1
• NCBI GenBank Nucleotide #: NP_001128476.1
• UniProt Accession #: O75953
• NCBI Official Full Name: dnaJ homolog subfamily B member 5 isoform 2
• NCBI Official Synonym Full Names: DnaJ heat shock protein family (Hsp40) member B5
• NCBI Official Symbol: DNAJB5
• NCBI Official Synonym Symbols: Hsc40
• NCBI Protein Information: dnaJ homolog subfamily B member 5
• UniProt Protein Name: DnaJ homolog subfamily B member 5
• Protein Family: DnaJ homolog subfamily
• UniProt Gene Name: DNAJB5
• UniProt Synonym Gene Names: HSC40; Hsc40
• UniProt Entry Name: DNJB5_HUMAN

NCBI Description

This gene encodes a member of the DNAJ heat shock protein 40 family of co-chaperone proteins. The encoded protein contains an N-terminal DNAJ domain and a C-terminal substrate binding domain but lacks the cysteine-rich domain found in other DNAJ family members. In mice, a multi-protein complex containing this protein, thioredoxin 1, and histone deacetylase 4, serves as a master negative regulator of cardiac hypertrophy. [provided by RefSeq, Mar 2017]

Uniprot Description

DNAJB5: Expressed under normal conditions, its expression can further be increased after various stress treatments. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Chaperone

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: nucleus; cytosol

Molecular Function: protein binding; chaperone binding; unfolded protein binding

Biological Process: protein folding; negative regulation of transcription from RNA polymerase II promoter; response to unfolded protein