GM-CSF active protein

Human GM-CSF

Cat. Num. AAA691787

• Gene Names: CSF2; GMCSF
• Reactivity: Human
• Purity: > 98% by SDS-PAGE & silver stain
• Synonyms: GM-CSF; Human GM-CSF; Recombinant Human Granulocyte Macrophage Colony-Stimulating Factor (GM-CSF); Colony-stimulating factor; GM-CSF active protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: > 98% by SDS-PAGE & silver stain
• Form/Format: Lyophilized
• Sequence: APARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQ EPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQI ITFESFKENLKDFLLVIPFDCWEPVQE
• Sequence Length: 127

• Endotoxin Level: < 0.1ng/ug of GM-CSF
• Buffer: PBS, pH7.2
• Preparation and Storage: The lyophilized powder although stable at room temperature for 3 weeks, is best stored desiccated at -20 degree C. Reconstituted GM-CSF should be stored in working aliquots at -20 degree C. For long term storage it is recommended to add a carrier protein (0.1% HAS or BSA).

Testing Data

Testing Data #2

Related Product Information for GM-CSF active protein

Recombinant human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), a 14,5 kDa protein consisting of 127 amino acid residues (Ala18-Glu144), is a potent species specific stimulator of bone marrow cells and several other cell types. GM-CSF was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages and eosinophils. GM-CSF has also been reported to have a functional role on non-hematopoietic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. GM-CSF is species specific and human GM-CSF has no biological effects on mouse cells. GM-CSF exerts its biological effects through binding to specific cell surface receptors. The high affinity receptors required for human GM-CSF signal transduction have been shown to be heterodimers consisting of a GM-CSF-specific alpha chain and a common beta chain that is shared by the high-affinity receptors for IL-3 and IL-5.

Product Categories/Family for GM-CSF active protein

Cytokines & Growth Factors

References

1. Martinez-Moczygemba and Huston (2003) J. Allergy Clin Immunol 112:653 2. Barreda et al, (2004) Dev Comp Immunol 28:509 3. Eksioglu et al, (2007) Exp Hematol 35:1163 4. Cao Y, (2007) J Clin Invest 117:2362 5. Fleetwood et al, (2005) Crit Rev Immunol 25:405 6. Diederichs et al, (1991) Science 254:1779 7. Gough et al, (1984) Nature 309:763.

NCBI and Uniprot Product Information

• NCBI GI #: 27437030
• NCBI GeneID: 1437
• NCBI Accession #: NP_0000749
• NCBI GenBank Nucleotide #: NM_000758
• UniProt Accession #: P04141; Q14CE8; Q2VPI8; Q8NFI6
• Molecular Weight: 14.5 kDa
• NCBI Official Full Name: granulocyte-macrophage colony-stimulating factor
• NCBI Official Synonym Full Names: colony stimulating factor 2 (granulocyte-macrophage)
• NCBI Official Symbol: CSF2
• NCBI Official Synonym Symbols: GMCSF
• NCBI Protein Information: granulocyte-macrophage colony-stimulating factor; CSF; molgramostin; sargramostim
• UniProt Protein Name: Granulocyte-macrophage colony-stimulating factor
• UniProt Gene Name: CSF2
• UniProt Synonym Gene Names: GMCSF; GM-CSF; CSF
• UniProt Entry Name: CSF2_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

Subunit structure: Monomer. The signaling GM-CSF receptor complex is a dodecamer of two head-to-head hexamers of two alpha, two beta, and two ligand subunits. Ref.13

Subcellular location: Secreted.

Polymorphism: Variant Ile-117 may be a risk factor for atopic asthma.

Pharmaceutical use: Available under the names Leukine (Immunex) and Leucomax (Novartis). Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.

Sequence similarities: Belongs to the GM-CSF family.