Rat natriuretic peptide precursor A ELISA Kit | NPPA elisa kit

Rat Atrial Natriuretic Peptide, ANP ELISA Kit

Cat. Num. AAA700086

• Reactivity: Rat
• Synonyms: natriuretic peptide precursor A; Rat Atrial Natriuretic Peptide; ANP ELISA Kit; ANF; ANP; ATFB6; CDD-ANF; PND; atrial natriuretic factor; atriopeptin; cardionatrin; prepronatriodilatin; NPPA elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat NPPA. No significant cross-reactivity or interference between Rat NPPA and analogues was observed.
• Sequence Length: 153

• Samples: Serum, plasma, cell culture supernates, tissue homogenates and Cell lysates
• Detection Range: 7.8 pg/ml-500 pg/ml
• Sensitivity: 1.95 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NPPA elisa kit

Introduction: Atrial natriuretic peptide (ANP), atrial natriuretic factor (ANF), or atriopeptin, is a polypeptide hormone involved in the homeostatic control of body water, sodium, and adiposity. It is released by atrial myocytes, muscle cells in the atria of the heart, in response to high blood pressure. ANP acts to reduce the water, sodium and adipose loads on the circulatory system, thereby reducing blood pressure. ANP is a 28 amino acid peptide with a 17 amino acid ring in the middle of the molecule. The ring is formed by a disulfide bond between two cysteine residues at positions 7 and 23. ANP is closely related to BNP (brain natriuretic peptide) and CNP (C-type natriuretic peptide) which all share the same amino acid ring. ANP was discovered in 1981 by a team in Ottawa led by Adolfo J. de Bold after they made the seminal observation that injection of atrial (but not ventricular) tissue extracts into rats caused copious natriuresis. ANP is produced, stored and released by atrial myocytes, 3 muscle cells in the atria of the heart. It is released in response to a variety of signals induced by hypervolaemia, exercise or caloric restriction. The hormone is constitutively expressed in the ventricle in response to stress induced by increased afterload (eg. increased ventricular pressure from aortic stenosis) or injury (eg. myocardial infarction). Neutral endopeptidase (NEP)is the enzyme that metabolizes natriuretic peptides. Several inhibitors of NEP are currently being developed to treat disorders ranging from hypertension to heart failure. Most of them are dual inhibitors. Omapatrilat (dual inhibitor of NEP and Angiotensin Converting Enzyme) developed by BMS did not receive FDA approval due to angioedema safety concerns. Other dual inhibitors of NEP with ACE / angiotensin receptor are currently being developed by pharmaceutical companies.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ANP. Standards or samples are then 4 added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for ANP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain ANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ANP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 126723777
• NCBI GeneID: 100009087
• NCBI Accession #: NP_001075731.1
• NCBI GenBank Nucleotide #: NM_001082262.1
• UniProt Accession #: P07500
• Molecular Weight: 16,843 Da
• NCBI Official Full Name: natriuretic peptides A
• NCBI Official Symbol: NPPA
• NCBI Protein Information: natriuretic peptides A; ANF; ANP; prepronatriodilatin; atrial natriuretic factor; atrial natriuretic peptide; natriuretic peptide precursor A
• UniProt Protein Name: Natriuretic peptides A
• UniProt Gene Name: NPPA
• UniProt Synonym Gene Names: ANF; ANP
• UniProt Entry Name: ANF_RABIT

Uniprot Description

Function: Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3