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Western Blot (WB) (Western blot analysis of DNA Polymerase alpha 1 expression in A549 (A); A431 (B); HeLa (C) whole cell lysates.)

Rabbit DNA Polymerase Alpha 1 Polyclonal Antibody

DNA Polymerase Alpha 1 Antibody

Applications
Western Blot, Immunoprecipitation
Purity
Immunogen Affinity Chromatography
Synonyms
DNA Polymerase Alpha 1; Polyclonal Antibody; DNA Polymerase Alpha 1 Antibody; Purified Polyclonal DNA Polymerase alpha 1 Antibody; Polyclonal DNA Polymerase alpha 1 antibody; Anti-DNA Polymerase alpha 1 antibody; POLA antibody; DNA polymerase alpha catalytic subunit antibody; DNA polymerase alpha catalytic subunit p180 antibody; anti-DNA Polymerase Alpha 1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of DNA Polymerase alpha 1 protein
Purity/Purification
Immunogen Affinity Chromatography
Form/Format
Supplied in liquid form in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3 with 30% Glycerol and 0.01% Sodium Azide
Applicable Applications for anti-DNA Polymerase Alpha 1 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:1000
IP: 1:10-1:100
Immunogen
DNA Polymerase alpha 1 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human DNA Polymerase alpha 1 as the immunogen
Preparation and Storage
Store at 4 degree C for short term storage. For long term, aliquot and store at -20 degree C. Avoid repeat freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of DNA Polymerase alpha 1 expression in A549 (A); A431 (B); HeLa (C) whole cell lysates.)

Western Blot (WB) (Western blot analysis of DNA Polymerase alpha 1 expression in A549 (A); A431 (B); HeLa (C) whole cell lysates.)
Related Product Information for anti-DNA Polymerase Alpha 1 antibody
DNA Polymerase alpha 1 plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1.
Product Categories/Family for anti-DNA Polymerase Alpha 1 antibody

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Product Notes

The DNA Polymerase Alpha 1 (Catalog #AAA5314845) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's DNA Polymerase Alpha 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:1000 IP: 1:10-1:100. Researchers should empirically determine the suitability of the DNA Polymerase Alpha 1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNA Polymerase Alpha 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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