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Western Blot (WB) (DMRTA2 Antibody (C-term) western blot analysis in PC-3 cell line, mouse testis and zebra fish brain tissue lysates (35ug/lane).This demonstrates the DMRTA2 antibody detected the DMRTA2 protein (arrow).)

Rabbit DMRTA2 Polyclonal Antibody | anti-DMRTA2 antibody

DMRTA2 Antibody (C-term)

Reactivity
Human, Mouse, Zebrafish (Predicted Reactivity: Bovine)
Applications
Immunofluorescence, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
DMRTA2; Polyclonal Antibody; DMRTA2 Antibody (C-term); Doublesex- and mab-3-related transcription factor A2; Doublesex- and mab-3-related transcription factor 5; DMRT5; anti-DMRTA2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Zebrafish (Predicted Reactivity: Bovine)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This DMRTA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 487-515 amino acids from the C-terminal region of human DMRTA2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.48 (varies by lot)
Sequence Positions
487-515
Sequence Length
542
Applicable Applications for anti-DMRTA2 antibody
Immunofluorescence (IF), ELISA (EIA), Western Blot (WB)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(DMRTA2 Antibody (C-term) western blot analysis in PC-3 cell line, mouse testis and zebra fish brain tissue lysates (35ug/lane).This demonstrates the DMRTA2 antibody detected the DMRTA2 protein (arrow).)

Western Blot (WB) (DMRTA2 Antibody (C-term) western blot analysis in PC-3 cell line, mouse testis and zebra fish brain tissue lysates (35ug/lane).This demonstrates the DMRTA2 antibody detected the DMRTA2 protein (arrow).)

Immunofluorescence (IF)

(Fluorescent confocal image of U251 cell stained with DMRTA2 Antibody (C-term). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with DMRTA2 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). DMRTA2 immunoreactivity is localized to Microtubules significantly.)

Immunofluorescence (IF) (Fluorescent confocal image of U251 cell stained with DMRTA2 Antibody (C-term). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with DMRTA2 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). DMRTA2 immunoreactivity is localized to Microtubules significantly.)
Related Product Information for anti-DMRTA2 antibody
DMRTA2 may be involved in sexual development.
Product Categories/Family for anti-DMRTA2 antibody
References
Ottolenghi, C., et al. Genomics 79(3):333-343(2002)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
53356
NCBI Official Full Name
doublesex- and mab-3-related transcription factor A2
NCBI Official Synonym Full Names
DMRT-like family A2
NCBI Official Symbol
DMRTA2
NCBI Protein Information
doublesex- and mab-3-related transcription factor A2
UniProt Protein Name
Doublesex- and mab-3-related transcription factor A2
UniProt Gene Name
DMRTA2
UniProt Synonym Gene Names
DMRT5
UniProt Entry Name
DMTA2_HUMAN

Uniprot Description

DMRTA2: May be involved in sexual development. Belongs to the DMRT family.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 1p32.3

Cellular Component: nucleus

Molecular Function: protein homodimerization activity; protein heterodimerization activity; metal ion binding; transcription factor activity

Biological Process: positive regulation of neuroblast proliferation; regulation of transcription, DNA-dependent; neuron fate specification; brain development; cerebral cortex regionalization; sex differentiation

Research Articles on DMRTA2

Similar Products

Product Notes

The DMRTA2 dmrta2 (Catalog #AAA9208127) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 487-515. The DMRTA2 Antibody (C-term) reacts with Human, Mouse, Zebrafish (Predicted Reactivity: Bovine) and may cross-react with other species as described in the data sheet. AAA Biotech's DMRTA2 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), ELISA (EIA), Western Blot (WB). WB~~1:1000. Researchers should empirically determine the suitability of the DMRTA2 dmrta2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DMRTA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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