anti-Human GNB3 & GNB4 Antibody | anti-GNB3 antibody

Goat anti-GNB3 & GNB4, Biotinylated Antibody

Cat. Num. AAA423748

• Gene Names: GNB3; CSNB1H
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: GNB3 & GNB4; Goat anti-GNB3 & GNB4; Biotinylated Antibody; Goat Anti-GNB3 & GNB4 Antibody; GNB3; guanine nucleotide binding protein (G protein); beta polypeptide 3; G protein; beta-3 subunit; GTP-binding regulatory protein beta-3 chain; guanine nucleotide-binding protein G(I)/G(S)/G(T) beta subunit 3; guanine nucleotide-binding protein; beta-3; anti-GNB3 antibody

• Reactivity: Human
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Sequence Length: 340

• Applicable Applications for anti-GNB3 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Pep-ELISA: antibody detection limit dilution 1:16000.; WB: Approx 35kDa band observed in lysates of cell line HepG2. Recommended concentration: 0.1-0.3ug/ml.
• Immunogen Sequence: Peptide with sequence SGHDNRVSCLGVT., from the internal region of the protein sequence according to NP_002066.1, NP_067642.1.
• Peptide Sequence + C: SGHDNRVSCLGVT.
• Epitope: Internal Region
• Note: This antibody is expected to recognize GNB3 & GNB4 (GeneID: 2784, 59345; ProteinID: NP_002066.1; NP_067642.1).
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Biotinylated (0.1ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)

NCBI and Uniprot Product Information

• NCBI GI #: 4504053
• NCBI GeneID: 2784
• NCBI Accession #: NP_002066.1; NP_067642.1
• NCBI GenBank Nucleotide #: NM_002075.3
• UniProt Accession #: P16520; Q96B71; Q9BQC0
• Molecular Weight: 37.2kDa
• NCBI Official Full Name: guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3 isoform 1
• NCBI Official Synonym Full Names: G protein subunit beta 3
• NCBI Official Symbol: GNB3
• NCBI Official Synonym Symbols: CSNB1H
• NCBI Protein Information: guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3
• UniProt Protein Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3
• UniProt Gene Name: GNB3

NCBI Description

Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit which belongs to the WD repeat G protein beta family. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. A single-nucleotide polymorphism (C825T) in this gene is associated with essential hypertension and obesity. This polymorphism is also associated with the occurrence of the splice variant GNB3-s, which appears to have increased activity. GNB3-s is an example of alternative splicing caused by a nucleotide change outside of the splice donor and acceptor sites. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2014]

Uniprot Description

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.