Western Blot (WB)
(All lanes : Anti-DKK3 Antibody (A30) at 1:1000 dilutionLane 1: 293T/17 whole cell lysateLane 2: A549 whole cell lysateLane 3: MCF-7 whole cell lysateLane 4: HL-60 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 38 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human, Mouse DKK3 Polyclonal Antibody | anti-DKK3 antibody
DKK3 Antibody (N-term)
Western Blot (WB)
(All lanes : Anti-DKK3 Antibody (A30) at 1:1000 dilutionLane 1: 293T/17 whole cell lysateLane 2: A549 whole cell lysateLane 3: MCF-7 whole cell lysateLane 4: HL-60 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 38 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-DKK3 Antibody (A30) at 1:1000 dilutionLane 1: 293T/17 whole cell lysateLane 2: A549 whole cell lysateLane 3: MCF-7 whole cell lysateLane 4: HL-60 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 38 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-DKK3 Antibody A30 at 1:1000 dilutionLane 1: 293T whole cell lysatesLane 2: A549 whole cell lysatesLane 3: HL-60 whole cell lysatesLane 4: mouse brain lysatesLane 5: MCF-7 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 38 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-DKK3 Antibody A30 at 1:1000 dilutionLane 1: 293T whole cell lysatesLane 2: A549 whole cell lysatesLane 3: HL-60 whole cell lysatesLane 4: mouse brain lysatesLane 5: MCF-7 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 38 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Background: DKK3, like DKK1, DKK2, and DKK4, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis revealed wide expression of the DKK3 transcript, with highest expression in heart, brain, and spinal cord. In situ hybridization reveals highest expression in mouse brain, eye, and heart.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
Uniprot Description
DKK3: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Belongs to the dickkopf family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 11p15.2
Cellular Component: extracellular space
Biological Process: anatomical structure morphogenesis; Wnt receptor signaling pathway; adrenal gland development; negative regulation of aldosterone biosynthetic process; negative regulation of transcription, DNA-dependent
Research Articles on DKK3
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Product Notes
The DKK3 dkk3 (Catalog #AAA9231945) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DKK3 Antibody (N-term) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DKK3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000. Researchers should empirically determine the suitability of the DKK3 dkk3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DKK3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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